Ultrastructural Imaging of Salmonella–Host Interactions Using Super‐resolution Correlative Light‐Electron Microscopy of Bioorthogonal Pathogens
Sign Up to like & getrecommendations! Published in 2018 at "Chembiochem"
DOI: 10.1002/cbic.201800230
Abstract: The imaging of intracellular pathogens inside host cells is complicated by the low resolution and sensitivity of fluorescence microscopy and by the lack of ultrastructural information to visualize the pathogens. Herein, we present a new… read more here.
Keywords: resolution; microscopy; correlative light; electron microscopy ... See more keywords
Applications of genetically encoded photosensitizer miniSOG: from correlative light electron microscopy to immunophotosensitizing.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biophotonics"
DOI: 10.1002/jbio.201600120
Abstract: Genetically encoded photosensitizers (PSs), e.g. ROS generating proteins, correspond to a novel class of PSs that are highly desirable for biological and medical applications since they can be used in combination with a variety of… read more here.
Keywords: microscopy; genetically encoded; correlative light; electron microscopy ... See more keywords
Labelling strategies for correlative light electron microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "Microscopy research and technique"
DOI: 10.1002/jemt.24304
Abstract: Imaging is one of the key technologies underpinning discoveries in biomedical research. Each imaging technique however usually only provides a specific type of information. For instance, live-cell imaging using fluorescent tags can show us the… read more here.
Keywords: electron microscopy; microscopy; labelling strategies; light electron ... See more keywords
Three‐dimensional fine structures of the maculae flavae of the human vocal fold using correlative light and electron microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Laryngoscope Investigative Otolaryngology"
DOI: 10.1002/lio2.950
Abstract: To analyze various aspects of complex tissue, there is increasing demand to study each sample at different length scales in biology. Correlative light and electron microscopy (CLEM) is the latest technique to correlate two different… read more here.
Keywords: electron microscopy; microscopy; light electron; three dimensional ... See more keywords
Correlative Light and Electron Microscopy for Nanoparticle-Cell Interaction and Protein Localization.
Sign Up to like & getrecommendations! Published in 2021 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-33-6064-8_6
Abstract: Various silica-based fluorescent nanoparticles ((Si-FNP)) with magnetic or metal cores represent a standard class of nanoparticles offering new opportunities for high-resolution cellular imaging and biomedicine applications, such as drug delivery. Their high solubility, homogeneity, biocompatibility,… read more here.
Keywords: microscopy; nanoparticle cell; electron microscopy; correlative light ... See more keywords
Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Science China Life Sciences"
DOI: 10.1007/s11427-018-9380-3
Abstract: Remarkable progress in correlative light and electron cryo-microscopy (cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope (cryo-FM). Here, we describe an ultra-stable super-resolution cryo-FM that… read more here.
Keywords: microscopy; super resolution; correlative light; cryo ... See more keywords
Find your coat: Using correlative light and electron microscopy to study intracellular protein coats.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2021.01.013
Abstract: Protein coats, important for vesicular trafficking in eukaryotic cells, help shape membranes and package cargo. But their dynamic construction cannot be fully understood until the distinct steps of their assembly in their native intracellular context… read more here.
Keywords: protein coats; microscopy; electron microscopy; correlative light ... See more keywords
A rapid workflow for the characterization of small numbers of unicellular eukaryotes by using correlative light and electron microscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2020.105888
Abstract: The limited availability of biological samples hinders phylogenetic efforts to define structural differences among various biological groups. A novel workflow enabling the analysis of protists in low cell numbers by electron microscopy (EM) is described… read more here.
Keywords: light electron; correlative light; electron microscopy; microscopy ... See more keywords
Correlative Light and 3D Electron Microscopy of Subnuclear Structures.
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927618006591
Abstract: Subnuclear structures such as the nucleolus have historically been studied mostly for histological interest. However, the pathological significance of the nucleolus has only been appreciated since the eighties. This was mainly due to the fact… read more here.
Keywords: microscopy; subnuclear structures; electron microscopy; correlative light ... See more keywords
Precise Protein Localization in Dissected Drosophila Larvae by Correlative Light and Transmission Electron Microscopy
Sign Up to like & getrecommendations! Published in 2018 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927618007341
Abstract: Bridging light and electron microscopy through correlative light and electron microscopy (CLEM) enables imaging of protein localization within a detailed subcellular context [1]. Certain CLEM approaches, such as array tomography, nanofEM, are compatible with super-resolution… read more here.
Keywords: microscopy; protein localization; correlative light; electron microscopy ... See more keywords
Structural Analysis of Gliding Motility of a Bacteroidetes Bacterium by Correlative Light and Scanning Electron Microscopy (CLSEM)
Sign Up to like & getrecommendations! Published in 2022 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927622000095
Abstract: Abstract The members of the Bacteroidetes phylum move on surfaces by gliding motility in the absence of external motility appendages, leading to the formation of spreading colonies. Here, the structural features of the spreading colony… read more here.
Keywords: microscopy; bacteroidetes bacterium; scanning electron; correlative light ... See more keywords