Development of a dual-antimicrobial counterselection method for markerless genetic engineering of bacterial genomes
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9565-5
Abstract: Markerless genetic engineering of bacterial genomes is commonly performed by two-step homologous recombination methods using vectors carrying flanking regions of the target gene for site-specific vector integration and counterselection markers to provide positive selection pressure… read more here.
Keywords: markerless genetic; method; selection; counterselection ... See more keywords
Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac649
Abstract: Abstract Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole… read more here.
Keywords: counterselection; markerless; integration; robust counterselection ... See more keywords
Markerless deletion of putative alanine dehydrogenase genes in Bacillus licheniformis using a codBA-based counterselection technique.
Sign Up to like & getrecommendations! Published in 2017 at "Microbiology"
DOI: 10.1099/mic.0.000544
Abstract: Bacillus licheniformis strains are used for the large-scale production of industrial exoenzymes from proteinaceous substrates, but details of the amino acid metabolism involved are largely unknown. In this study, two chromosomal genes putatively involved in… read more here.
Keywords: alanine; markerless; bacillus licheniformis; counterselection ... See more keywords
Quantifying immune-based counterselection of somatic mutations
Sign Up to like & getrecommendations! Published in 2019 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1008227
Abstract: Somatic mutations in protein-coding regions can generate ‘neoantigens’ causing developing cancers to be eliminated by the immune system. Quantitative estimates of the strength of this counterselection phenomenon have been lacking. We quantified the extent to… read more here.
Keywords: based counterselection; counterselection somatic; somatic mutations; counterselection ... See more keywords