Efficient markerless gene deletions in Pseudomonas protegens Pf-5 using a upp-based counterselective system
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DOI: 10.1007/s10529-019-02772-5
Abstract: ObjectivesDeveloping a counterselective system for efficient markerless gene deletions in biocontrol strain P. protegens Pf-5.ResultsWe successfully implemented a markerless deletion of upp in Pf-5 to obtain the 5-FU resistant strain Pf5139. With this strain, we… read more here.
Keywords: gene deletions; gene; markerless gene; counterselective system ... See more keywords