Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity
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DOI: 10.1039/c9ay02283j
Abstract: A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I. In this assay, a double-stranded DNA… read more here.
Keywords: detection; coupled exonuclease; fluorescence; pnk ... See more keywords