Reconstitution and functional characterization of SARS-CoV-2 proofreading complex
Sign Up to like & getrecommendations! Published in 2021 at "Protein Expression and Purification"
DOI: 10.1016/j.pep.2021.105894
Abstract: The novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2 or COVID-19) has led to a world-wild pandemic. The replication of SARS-CoV-2 RNA genome involves the core replication-transcription complex (RTC, nsp12-nsp7-nsp8) and the proofreading complex (nsp14-nsp10)… read more here.
Keywords: reconstitution functional; proofreading complex; sars cov; cov nsp14 ... See more keywords
Potent Inhibition of SARS-CoV-2 nsp14 N7-Methyltransferase by Sulfonamide-Based Bisubstrate Analogues
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medicinal Chemistry"
DOI: 10.1021/acs.jmedchem.2c00120
Abstract: Enzymes involved in RNA capping of SARS-CoV-2 are essential for the stability of viral RNA, translation of mRNAs, and virus evasion from innate immunity, making them attractive targets for antiviral agents. In this work, we… read more here.
Keywords: nsp14 methyltransferase; inhibition sars; cov nsp14; sars cov ... See more keywords
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein
Sign Up to like & getrecommendations! Published in 2021 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2101161118
Abstract: Significance To establish infection, pathogenic viruses have to overcome the type I interferon (IFN-I) antiviral response. A previous study demonstrated that the SARS-CoV-2 NSP14 is able to inhibit IFN-I responses. In this study, we report… read more here.
Keywords: translation; host; sars cov; innate immune ... See more keywords
Crystal structures and fragment screening of SARS-CoV-2 NSP14 reveal details of exoribonuclease activation and mRNA capping and provide starting points for antiviral drug development
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1207
Abstract: The SARS-CoV-2 non-structural protein 14 (NSP14) is a dual function enzyme containing an N-terminal exonuclease domain (ExoN) and C-terminal Guanine-N7-methyltransferase (N7-MTase) domain. Both enzymatic activities appear to be essential for the viral life cycle and… read more here.
Keywords: reveal; structure; mrna capping; fragment screening ... See more keywords
SARS-CoV-2 Nsp14 protein associates with IMPDH2 and activates NF-κB signaling
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1007089
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leads to NF-κB activation and induction of pro-inflammatory cytokines, though the underlying mechanism for this activation is not fully understood. Our results reveal that the SARS-CoV-2 Nsp14… read more here.
Keywords: impdh2; nsp14 protein; cov nsp14; activation ... See more keywords