Strategies for Covalent Labeling of Long RNAs
Sign Up to like & getrecommendations! Published in 2021 at "Chembiochem"
DOI: 10.1002/cbic.202100161
Abstract: The introduction of chemical modifications into long RNA molecules at specific positions for visualization, biophysical investigations, diagnostic and therapeutic applications still remains challenging. In this review, we present recent approaches for covalent internal labeling of… read more here.
Keywords: strategies covalent; covalent labeling; long rnas; biology ... See more keywords
Distinguishing Histidine Tautomers in Proteins Using Covalent Labeling-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03902
Abstract: In this work, we use diethylpyrocarbonate (DEPC)-based covalent labeling together with LC-MS/MS analysis to distinguish the two sidechain tautomers of histidine residues in peptides and proteins. From labeling experiments on model peptides, we demonstrate that… read more here.
Keywords: histidine residues; tautomers proteins; covalent labeling; histidine tautomers ... See more keywords
Membrane Protein Binding Interactions Studied in Live Cells via Diethylpyrocarbonate Covalent Labeling Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05616
Abstract: Membrane proteins are vital in the human proteome for their cellular functions and make up a majority of drug targets in the U.S. However, characterizing their higher-order structures and interactions remains challenging. Most often membrane… read more here.
Keywords: labeling mass; mass spectrometry; covalent labeling; membrane ... See more keywords
Using Covalent Labeling and Mass Spectrometry To Study Protein Binding Sites of Amyloid Inhibiting Molecules.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b02915
Abstract: Amyloid aggregates are associated with several debilitating diseases, and there are numerous efforts to develop small molecule treatments against these diseases. One challenge associated with these efforts is determining protein binding site information for potential… read more here.
Keywords: small molecule; binding sites; protein binding; covalent labeling ... See more keywords
Quantitative Analysis of Protein Covalent Labeling Mass Spectrometry Data in the Mass Spec Studio.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b01625
Abstract: Covalent labeling with mass spectrometry (CL-MS) provides a direct measure of the chemical and structural features of proteins with the potential for resolution at the amino-acid level. Unfortunately, most applications of CL-MS are limited to… read more here.
Keywords: mass spectrometry; covalent labeling; analysis; mass ... See more keywords
Rapid Covalent Labeling of Membrane Proteins on Living Cells Using a Nanobody-Epitope Tag Pair.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.2c00334
Abstract: Synthetic molecules that form a covalent bond upon binding to a targeted biomolecule (proximity-induced reactivity) are the subject of intense biomedical interest for the unique pharmacological properties imparted by irreversible binding. However, off-target covalent labeling… read more here.
Keywords: epitope; nanobody; cross linking; covalent labeling ... See more keywords
Protein complex prediction using Rosetta, AlphaFold, and mass spectrometry covalent labeling
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1101/2022.04.30.490108
Abstract: Covalent labeling (CL) in combination with mass spectrometry can be used as an analytical tool to study and determine structural properties of protein-protein complexes. However, data from these experiments is sparse and does not unambiguously… read more here.
Keywords: mass spectrometry; protein complex; covalent labeling; protein ... See more keywords