Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems
Sign Up to like & getrecommendations! Published in 2018 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-018-2810-3
Abstract: CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only in human cells and… read more here.
Keywords: genome editing; system; mammalian systems; crispr cpf1 ... See more keywords
CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
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DOI: 10.1007/s00299-017-2118-z
Abstract: Key messageCRISPR-Cas9/Cpf1 system with its unique gene targeting efficiency, could be an important tool for functional study of early developmental genes through the generation of successful knockout plants.AbstractThe introduction and utilization of systems biology approaches… read more here.
Keywords: crispr; crispr cas9; cpf1; biology ... See more keywords
CRISPR/Cpf1-mediated mutagenesis and gene deletion in industrial filamentous fungi Aspergillus oryzae and Aspergillus sojae.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2021.12.017
Abstract: In industrial applications such as fermentation and heterologous protein production, various Aspergillus oryzae and A. sojae strains are used. Although genetic engineering techniques have been developed for these filamentous fungi, applying such classical techniques to… read more here.
Keywords: genetic engineering; sojae; genome editing; cpf1 ... See more keywords
Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00054
Abstract: Mismatch tolerance, a cause of the off-target effect, impedes accurate genome editing with the CRISPR/Cas system. Herein, we observed that oligonucleotide-directed single-base substitutions could be rarely introduced in the microbial genome using CRISPR/Cpf1-mediated negative selection.… read more here.
Keywords: genome editing; microbial genome; genome; end truncated ... See more keywords
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
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DOI: 10.1038/ncomms15179
Abstract: Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into… read more here.
Keywords: genome editing; cpf1 assisted; crispr cpf1; cpf1 ... See more keywords
Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors
Sign Up to like & getrecommendations! Published in 2017 at "Nature methods"
DOI: 10.1038/nmeth.4483
Abstract: Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to tune the expression of endogenous genes in human cells.… read more here.
Keywords: gene regulation; multiplex gene; cpf1; gene ... See more keywords
Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03760-5
Abstract: Human pluripotent stem cells (hPSCs) have potential applications in biological studies and regenerative medicine. However, precise genome editing in hPSCs remains time-consuming and labor-intensive. Here we demonstrate that the recently identified CRISPR-Cpf1 can be used… read more here.
Keywords: genome editing; human pluripotent; small molecules; crispr cpf1 ... See more keywords
Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1
Sign Up to like & getrecommendations! Published in 2018 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btx695
Abstract: Summary Following the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of… read more here.
Keywords: genome wide; crispr; cpf1; cpf1 database ... See more keywords
Prediction of activity and specificity of CRISPR-Cpf1 using convolutional deep learning neural networks
Sign Up to like & getrecommendations! Published in 2019 at "BMC Bioinformatics"
DOI: 10.1186/s12859-019-2939-6
Abstract: BackgroundCRISPR-Cpf1 has recently been reported as another RNA-guided endonuclease of class 2 CRISPR-Cas system, which expands the molecular biology toolkit for genome editing. However, most of the online tools and applications to date have been… read more here.
Keywords: activity specificity; crispr; neural networks; cpf1 ... See more keywords