CRAGE-CRISPR facilitates rapid activation of secondary metabolite biosynthetic gene clusters in bacteria.
Sign Up to like & getrecommendations! Published in 2021 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2021.08.009
Abstract: With the advent of genome sequencing and mining technologies, secondary metabolite biosynthetic gene clusters (BGCs) within bacterial genomes are becoming easier to predict. For subsequent BGC characterization, clustered regularly interspaced short palindromic repeats (CRISPR) has… read more here.
Keywords: crage; secondary metabolite; crage crispr; biosynthetic gene ... See more keywords
CRAGE-Duet facilitates modular assembly of biological systems for studying plant-microbe interactions.
Sign Up to like & getrecommendations! Published in 2020 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.0c00280
Abstract: Developing sustainable agricultural practices will require increasing our understanding of plant-microbe interactions. To study these interactions, new genetic tools for manipulating non-model microbes will be needed. To help meet this need, we recently reported development… read more here.
Keywords: microbe interactions; crage; plant microbe; crage duet ... See more keywords