Inserting Cre recombinase into the Prolactin 8a2 gene for decidua‐specific recombination in mice
Sign Up to like & getrecommendations! Published in 2022 at "genesis"
DOI: 10.1002/dvg.23473
Abstract: An estimated 75% of unsuccessful pregnancies are due to implantation failure. Investigating the causes of implantation failure is difficult as decidualization and embryo implantation is a dynamic process. Here, we describe a new decidua‐specific iCre… read more here.
Keywords: decidua specific; icre recombinase; inserting cre; recombinase ... See more keywords
Cre Recombinase Strains Used for the Study of Adipose Tissues and Adipocyte Progenitors
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25675
Abstract: Adipose tissues play important roles in whole body energy homeostasis and lifer span. Understanding the mechanisms of controlling adipose tissues development is significant for providing useful information to treat the worldwide epidemic of obesity and… read more here.
Keywords: tissues adipocyte; study adipose; strains used; adipose tissues ... See more keywords
Confounding influence of tamoxifen in mouse models of Cre recombinase-induced gene activity or modulation
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Toxicology"
DOI: 10.1007/s00204-018-2254-4
Abstract: Tamoxifen (TAM) is commonly used for cell type specific Cre recombinase-induced gene inactivation and in cell fate tracing studies. Inducing a gene knockout by TAM and using non-TAM exposed mice as controls lead to a… read more here.
Keywords: induced gene; tamoxifen; recombinase induced; cre recombinase ... See more keywords
Mechanisms of Cre recombinase synaptic complex assembly and activation illuminated by Cryo-EM
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac032
Abstract: Abstract Cre recombinase selectively recognizes DNA and prevents non-specific DNA cleavage through an orchestrated series of assembly intermediates. Cre recombines two loxP DNA sequences featuring a pair of palindromic recombinase binding elements and an asymmetric… read more here.
Keywords: mechanisms cre; assembly; synaptic complex; cre recombinase ... See more keywords
Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz585
Abstract: Abstract A common mechanism for inducibly controlling protein function relies on reconstitution of split protein fragments using chemical or light-induced dimerization domains. A protein is split into fragments that are inactive on their own, but… read more here.
Keywords: protein; control; recombinase; split ... See more keywords
Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Neuroscience Methods"
DOI: 10.1101/2022.04.13.488137
Abstract: Background Cell groups containing catecholamines provide a useful model to study the molecular and cellular mechanisms underlying the morphogenesis, physiology, and pathology of the central nervous system. For this purpose, it is necessary to establish… read more here.
Keywords: physiology; cre recombinase; specific expression; expression ... See more keywords
Tamoxifen administration induces histopathologic changes within the lungs of Cre-recombinase-negative mice: A case report.
Sign Up to like & getrecommendations! Published in 2021 at "Laboratory animals"
DOI: 10.1177/00236772211042968
Abstract: Tamoxifen is commonly used as a cancer treatment in humans and for inducing genetic alterations using Cre-lox mouse models in the research setting. However, the extent of tamoxifen off-target effects in animal research is underappreciated.… read more here.
Keywords: recombinase negative; negative mice; tamoxifen; cre recombinase ... See more keywords
Viral Cre-LoxP tools aid genome engineering in mammalian cells
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biological Engineering"
DOI: 10.1186/s13036-017-0087-y
Abstract: BackgroundTargeted nucleases have transformed genome editing technology, providing more efficient methods to make targeted changes in mammalian genome. In parallel, there is an increasing demand of Cre-LoxP technology for complex genome manipulation such as large… read more here.
Keywords: engineering; cre recombinase; cre loxp; mammalian cells ... See more keywords
Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus
Sign Up to like & getrecommendations! Published in 2020 at "eNeuro"
DOI: 10.1523/eneuro.0433-19.2020
Abstract: Abstract The mu-opioid receptor (MOR) modulates nociceptive pathways and reward processing, and mediates the strong analgesic and addictive properties of both medicinal as well as abused opioid drugs. MOR function has been extensively studied, and… read more here.
Keywords: receptor; line; cre; mor ... See more keywords
Generation of a Gal4-dependent gene recombination and illuminating mouse
Sign Up to like & getrecommendations! Published in 2022 at "Experimental Animals"
DOI: 10.1538/expanim.21-0202
Abstract: Cell labeling technologies, including the Cre/loxP system, are powerful tools in developmental biology. Although the conventional Cre/loxP system has been extensively used to label the expression of specific genes, it is less frequently used for… read more here.
Keywords: gal4 dependent; cre recombinase; mouse; mouse line ... See more keywords
Cre-loxP Reporter Mouse Reveals Stochastic Activity of the Foxp3 Promoter
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2019.02228
Abstract: Mouse models that combine specific loxP-flanked gene sequences with Cre recombinase expressed from cell-regulated promoters have become important tools to investigate gene function. Critically however, expression of Cre recombinase may not always be restricted to… read more here.
Keywords: reporter; cre recombinase; promoter; gene ... See more keywords