Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
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DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo
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DOI: 10.1002/advs.201801423
Abstract: Abstract CRISPR/dCas9 systems can precisely control endogenous gene expression without interrupting host genomic sequence and have provided a novel and feasible strategy for the treatment of cancers at the transcriptional level. However, development of CRISPR/dCas9‐based… read more here.
Keywords: crispr; vivo; delivery; crispr dcas9 ... See more keywords
CRISPR‐Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids
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DOI: 10.1002/advs.202206872
Abstract: CRISPR/Cas systems offer a powerful sensing mechanism to transduce sequence‐specific information into amplified analytical signals. However, performing multiplexed CRISPR/Cas assays remains challenging and often requires complex approaches for multiplexed assays. Here, a hydrogel‐based CRISPR/Cas12 system… read more here.
Keywords: crispr; enhanced hydrogel; crispr enhanced; detection ... See more keywords
A CRISPR view of hematopoietic stem cells: Moving innovative bioengineering into the clinic
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DOI: 10.1002/ajh.26588
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas genome engineering has emerged as a powerful tool to modify precise genomic sequences with unparalleled accuracy and efficiency. Major advances in CRISPR technologies over the last 5 years have… read more here.
Keywords: crispr; view hematopoietic; hematopoietic stem; stem cells ... See more keywords
Potential of the CRISPR‐Cas system for improved parasite diagnosis
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DOI: 10.1002/bies.202100286
Abstract: CRISPR‐Cas technology accelerates development of fast, accurate, and portable diagnostic tools, typified by recent applications in COVID‐19 diagnosis. Parasitic helminths cause devastating diseases afflicting 1.5 billion people globally, representing a significant public health and economic… read more here.
Keywords: system; crispr; diagnosis; crispr cas ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
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DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
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DOI: 10.1002/biot.201700601
Abstract: Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular… read more here.
Keywords: engineering; saccharomyces cerevisiae; crispr; system ... See more keywords
Breakthrough in CRISPR/Cas system: Current and future directions and challenges.
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DOI: 10.1002/biot.202200642
Abstract: Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists to develop desirable varieties. This technology involves inducing site-specific DNA perturbations that can be repaired… read more here.
Keywords: genome editing; technology; crispr cas; crispr ... See more keywords
Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum
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DOI: 10.1002/bit.27753
Abstract: Clostridium butyricum has been widely used as a probiotic for humans and food animals. However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly understood, largely due to the lack of… read more here.
Keywords: genome editing; butyricum; crispr; crispr cas ... See more keywords
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches
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DOI: 10.1002/bit.28344
Abstract: Vegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make them an important part of our diet. Numerous biotic and abiotic stresses can affect crop growth, quality, and yield. Traditional and… read more here.
Keywords: genome editing; crop improvement; crispr;
Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
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DOI: 10.1002/bit.28367
Abstract: The production of high‐value biopharmaceuticals is dominated by mammalian production cells, particularly Chinese hamster ovary (CHO) cells, which have been widely used and preferred in manufacturing processes. The discovery of CRISPR‐Cas9 significantly accelerated cell line… read more here.
Keywords: genome editing; crispr toolbox; crispr; chinese hamster ... See more keywords