The anti-CRISPR protein AcrIE8.1 inhibits the type I-E CRISPR-Cas system by directly binding to the Cascade subunit Cas11.
Sign Up to like & getrecommendations! Published in 2025 at "FEBS letters"
DOI: 10.1002/1873-3468.70201
Abstract: CRISPR-Cas systems provide adaptive immunity to bacteria by recognizing and destroying foreign genetic elements. The type I-E CRISPR-Cas system utilizes a multi-subunit Cascade complex to detect target DNA and recruit the Cas3 nuclease for degradation.… read more here.
Keywords: crispr; cas system; subunit; crispr cas ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
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DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo
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DOI: 10.1002/advs.201801423
Abstract: Abstract CRISPR/dCas9 systems can precisely control endogenous gene expression without interrupting host genomic sequence and have provided a novel and feasible strategy for the treatment of cancers at the transcriptional level. However, development of CRISPR/dCas9‐based… read more here.
Keywords: crispr; vivo; delivery; crispr dcas9 ... See more keywords
CRISPR‐Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids
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DOI: 10.1002/advs.202206872
Abstract: CRISPR/Cas systems offer a powerful sensing mechanism to transduce sequence‐specific information into amplified analytical signals. However, performing multiplexed CRISPR/Cas assays remains challenging and often requires complex approaches for multiplexed assays. Here, a hydrogel‐based CRISPR/Cas12 system… read more here.
Keywords: crispr; enhanced hydrogel; crispr enhanced; detection ... See more keywords
Magnetic Nanoparticle‐Assisted Non‐Viral CRISPR‐Cas9 for Enhanced Genome Editing to Treat Rett Syndrome
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DOI: 10.1002/advs.202306432
Abstract: The CRISPR‐Cas9 technology has the potential to revolutionize the treatment of various diseases, including Rett syndrome, by enabling the correction of genes or mutations in human patient cells. However, several challenges need to be addressed… read more here.
Keywords: crispr; rett syndrome; genome editing; magnetic nanoparticle ... See more keywords
A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules.
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DOI: 10.1002/advs.202503917
Abstract: Epigenome and cis‐regulome, comprising cis‐regulatory elements (CREs) and modules (CRMs), jointly define the architecture of gene regulation. However, the causal mechanisms by which epigenetic marks influence CRM function remain elusive. To address this, modular epigenome… read more here.
Keywords: epigenome editing; methylation; cis regulatory; logic ... See more keywords
Enhanced One‐Pot Dual‐CRISPR‐Based Assay Lyophilized on a 3D‐Printed Disc for Field‐Deployable Multiplex Bacteria Detection
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DOI: 10.1002/advs.202509355
Abstract: One‐pot CRISPR‐based detection combining recombinase polymerase amplification (RPA) enables rapid and accurate nucleic acid testing but faces challenges in performance, multiplexing, and field‐ready lyophilization. Here, an enhanced one‐pot, helicase‐assisted RPA (hRPA)‐combined, dual‐CRISPR/uAsCas12a (EOD‐CRISPR) assay is… read more here.
Keywords: detection; one pot; eod crispr; crispr ... See more keywords
A CRISPR view of hematopoietic stem cells: Moving innovative bioengineering into the clinic
Sign Up to like & getrecommendations! Published in 2022 at "American Journal of Hematology"
DOI: 10.1002/ajh.26588
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas genome engineering has emerged as a powerful tool to modify precise genomic sequences with unparalleled accuracy and efficiency. Major advances in CRISPR technologies over the last 5 years have… read more here.
Keywords: crispr; view hematopoietic; hematopoietic stem; stem cells ... See more keywords
Potential of the CRISPR‐Cas system for improved parasite diagnosis
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DOI: 10.1002/bies.202100286
Abstract: CRISPR‐Cas technology accelerates development of fast, accurate, and portable diagnostic tools, typified by recent applications in COVID‐19 diagnosis. Parasitic helminths cause devastating diseases afflicting 1.5 billion people globally, representing a significant public health and economic… read more here.
Keywords: system; crispr; diagnosis; crispr cas ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
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DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System
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DOI: 10.1002/biot.201700601
Abstract: Thanks to its ease of use, modularity, and scalability, the clustered regularly interspaced short palindromic repeats (CRISPR) system has been increasingly used in the design and engineering of Saccharomyces cerevisiae, one of the most popular… read more here.
Keywords: engineering; saccharomyces cerevisiae; crispr; system ... See more keywords