Endosomal Escapable and Nuclear Localizing Cationic Polyaspartate-Based CRISPR Activation System for Preventing Respiratory Virus Infection by Specifically Inducing Interferon-λ.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c16588
Abstract: Global pandemics caused by viruses cause widespread panic and economic losses. The lack of specific antivirals and vaccines increases the spreading of viral diseases worldwide. Thus, alternative strategies are required to manage viral outbreaks. Here,… read more here.
Keywords: system; respiratory virus; virus infection; crispr activation ... See more keywords
A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-71648-w
Abstract: Recent advances in CRISPR/Cas9 based genome editing have considerably advanced genetic engineering of industrial yeast strains. In this study, we report the construction and characterization of a toolkit for CRISPR activation and interference (CRISPRa/i) for… read more here.
Keywords: activation interference; toolkit; activation; strain ... See more keywords
Development of a CRISPR activation system for targeted gene upregulation in Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2024 at "Communications Biology"
DOI: 10.1038/s42003-025-08164-y
Abstract: The photosynthetic cyanobacterium Synechocystis sp. PCC 6803 offers a promising sustainable solution for simultaneous CO2 fixation and compound bioproduction. While various heterologous products have now been synthesised in Synechocystis, limited genetic tools hinder further strain… read more here.
Keywords: system; pcc 6803; synechocystis; crispr activation ... See more keywords
CRISPR activation to repair electrocardiogram abnormalities caused by a FLNC truncating variant in mice
Sign Up to like & getrecommendations! Published in 2025 at "European Heart Journal"
DOI: 10.1093/eurheartj/ehaf703
Abstract: BACKGROUND Truncating variants in the Filamin C gene (FLNCtv) are a frequent cause of genetic dilated cardiomyopathy (DCM) and non-dilated left ventricular cardiomyopathy (NDLVC), both characterized by arrhythmic complications and increased risk of sudden cardiac… read more here.
Keywords: mice; activation; crispr activation; expression ... See more keywords
CRISPR Activation Screen for HDAC Inhibitor Resistance
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DOI: 10.1182/blood-2018-99-119044
Abstract: Panobinostat is an oral broad-spectrum histone deacetylase inhibitor that alters gene expression via epigenetic mechanisms and function of key proteins through changes in their protein acetylation state. Panobinostate was approved a few years ago by… read more here.
Keywords: crispr activation; panobinostat; resistance; hdac ... See more keywords
A genome–wide CRISPR activation screen identifies SCREEM a novel SNAI1 super-enhancer demarcated by eRNAs
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1110445
Abstract: The genome is pervasively transcribed to produce a vast array of non-coding RNAs (ncRNAs). Long non-coding RNAs (lncRNAs) are transcripts of >200 nucleotides and are best known for their ability to regulate gene expression. Enhancer… read more here.
Keywords: crispr activation; genome wide; snai1; wide crispr ... See more keywords
Identification of NRAS Downstream Genes with CRISPR Activation Screening
Sign Up to like & getrecommendations! Published in 2022 at "Biology"
DOI: 10.3390/biology11111551
Abstract: Simple Summary NRAS mutations constitutively activate cell proliferation signaling in malignant tumors. The elucidation of NRAS downstream signaling genes may lead to the control of NRAS-mutant tumors. Genome-wide CRISPR activation screening was performed on THP-1… read more here.
Keywords: cell; proliferation; crispr activation; dohh hist1h2ac ... See more keywords