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Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz072
Abstract: Abstract Fine-tuning of gene expression is crucial for protein expression and pathway construction, but it still faces formidable challenges due to the hierarchical gene regulation at multiple levels in a context-dependent manner. In this study,…
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Keywords:
fine tuning;
tuning gene;
gene expression;
crispr assisted ... See more keywords
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Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14075
Abstract: Pseudomonas chlororaphis is a non‐pathogenic, plant growth‐promoting rhizobacterium that secretes phenazine compounds with broad‐spectrum antibiotic activity. Currently available genome‐editing methods for P. chlororaphis are based on homologous recombination (HR)‐dependent allelic exchange, which requires both exogenous…
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Keywords:
system;
crispr assisted;
pseudomonas chlororaphis;
base editing ... See more keywords
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Published in 2019 at "eLife"
DOI: 10.7554/elife.45973
Abstract: The deactivated CRISPR/Cas9 (dCas9) is now the most widely used gene activator. However, current dCas9-based gene activators are still limited by their unsatisfactory activity. In this study, we developed a new strategy, the CRISPR-assisted trans…
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Keywords:
assisted trans;
trans enhancer;
dcas9;
crispr assisted ... See more keywords