CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz072
Abstract: Abstract Fine-tuning of gene expression is crucial for protein expression and pathway construction, but it still faces formidable challenges due to the hierarchical gene regulation at multiple levels in a context-dependent manner. In this study,… read more here.
Keywords: fine tuning; tuning gene; gene expression; crispr assisted ... See more keywords
Developing a CRISPR‐assisted base‐editing system for genome engineering of Pseudomonas chlororaphis
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14075
Abstract: Pseudomonas chlororaphis is a non‐pathogenic, plant growth‐promoting rhizobacterium that secretes phenazine compounds with broad‐spectrum antibiotic activity. Currently available genome‐editing methods for P. chlororaphis are based on homologous recombination (HR)‐dependent allelic exchange, which requires both exogenous… read more here.
Keywords: system; crispr assisted; pseudomonas chlororaphis; base editing ... See more keywords
Gene activation by a CRISPR-assisted trans enhancer
Sign Up to like & getrecommendations! Published in 2019 at "eLife"
DOI: 10.7554/elife.45973
Abstract: The deactivated CRISPR/Cas9 (dCas9) is now the most widely used gene activator. However, current dCas9-based gene activators are still limited by their unsatisfactory activity. In this study, we developed a new strategy, the CRISPR-assisted trans… read more here.
Keywords: assisted trans; trans enhancer; dcas9; crispr assisted ... See more keywords