Discovery of Oligonucleotide Signaling Mediated by CRISPR-Associated Polymerases Solves Two Puzzles but Leaves an Enigma.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00713
Abstract: The signature component of type III CRISPR-Cas systems is the Cas10 protein that consists of two Palm domains homologous to those of DNA and RNA polymerases and nucleotide cyclases and an HD nuclease domain. However,… read more here.
Keywords: crispr; crispr cas; biology; crispr associated ... See more keywords
Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers
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DOI: 10.1038/nature23467
Abstract: In many prokaryotes, type III clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated (Cas) systems detect and degrade invasive genetic elements by an RNA-guided, RNA-targeting multisubunit interference complex. The CRISPR-associated protein Csm6 additionally contributes to interference… read more here.
Keywords: cyclic oligoadenylate; crispr; interference; cas systems ... See more keywords
Functional characterization of diverse type I-F CRISPR-associated transposons
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac985
Abstract: Abstract CRISPR-Cas systems generally provide adaptive immunity in prokaryotes through RNA-guided degradation of foreign genetic elements like bacteriophages and plasmids. Recently, however, transposon-encoded and nuclease-deficient CRISPR-Cas systems were characterized and shown to be co-opted by… read more here.
Keywords: crispr; type crispr; rna guided; crispr cas ... See more keywords
Salinarimonas soli sp. nov., isolated from soil.
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DOI: 10.1099/ijsem.0.005095
Abstract: A light pink coloured bacterium, designated strain BN140002T, was isolated from a soil sample collected in Goesan-gun, Chungcheongbuk-do, Republic of Korea. Cells of strain BN140002T were Gram-stain-negative, aerobic, motile and rod-shaped. Phylogenetic analysis based on… read more here.
Keywords: soli nov; salinarimonas soli; crispr; strain bn140002t ... See more keywords
RNA-guided DNA insertion with CRISPR-associated transposases
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DOI: 10.1126/science.aax9181
Abstract: Beyond adaptive immunity Prokaryotic CRISPR-Cas systems defend bacterial cells from phage and plasmid infection. Strecker et al. characterized a CRISPR-Cas system that functions beyond adaptive immunity (see the Perspective by Hou and Zhang). Type V-K… read more here.
Keywords: crispr; dna; insertion; rna guided ... See more keywords
RNA-activated protein cleavage with a CRISPR-associated endopeptidase
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.add7450
Abstract: In prokaryotes, CRISPR-Cas systems provide adaptive immune responses against foreign genetic elements through RNA-guided nuclease activity. Recently, additional genes with non-nuclease functions have been found in genetic association with CRISPR systems, suggesting that there may… read more here.
Keywords: rna activated; rna guided; crispr; activated protein ... See more keywords