Massively Parallel CRISPR‐Based Genetic Perturbation Screening at Single‐Cell Resolution
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202204484
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR)‐based genetic screening has been demonstrated as a powerful approach for unbiased functional genomics research. Single‐cell CRISPR screening (scCRISPR) techniques, which result from the combination of single‐cell toolkits… read more here.
Keywords: cell; resolution; single cell; based genetic ... See more keywords
Lrp5 Mutant and Crispant Zebrafish Faithfully Model Human Osteoporosis, Establishing the Zebrafish as a Platform for CRISPR‐Based Functional Screening of Osteoporosis Candidate Genes
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Bone and Mineral Research"
DOI: 10.1002/jbmr.4327
Abstract: Genomewide association studies (GWAS) have improved our understanding of the genetic architecture of common complex diseases such as osteoporosis. Nevertheless, to attribute functional skeletal contributions of candidate genes to osteoporosis‐related traits, there is a need… read more here.
Keywords: crispr based; osteoporosis candidate; candidate genes; osteoporosis ... See more keywords
Current progress in CRISPR‐based diagnostic platforms
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.27690
Abstract: The CRISPR‐Cas system is a key technology for genome editing and regulation in a wide range of organisms and cell types. Recently, CRISPR‐Cas–based diagnostic platform has shown idealistic properties for pathogen detection. Integrating the CRISPR‐Cas… read more here.
Keywords: progress crispr; crispr; current progress; crispr based ... See more keywords
A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1720-5_8
Abstract: Cyanobacteria, a group of diverse bacteria capable of oxygenic photosynthesis, are excellent models for investigating many important cellular processes, such as photosynthesis, nitrogen fixation, and prokaryotic cell differentiation. They also have great potential to become… read more here.
Keywords: crispr based; method constructing; method; essential genes ... See more keywords
CRISPR-based strategies for studying regulatory elements and chromatin structure in mammalian gene control
Sign Up to like & getrecommendations! Published in 2017 at "Mammalian Genome"
DOI: 10.1007/s00335-017-9727-2
Abstract: The development of high-throughput methods has enabled the genome-wide identification of putative regulatory elements in a wide variety of mammalian cells at an unprecedented resolution. Extensive genomic studies have revealed the important role of regulatory… read more here.
Keywords: based strategies; crispr based; chromatin; strategies studying ... See more keywords
Futuristic CRISPR-based biosensing in the cloud and internet of things era: an overview
Sign Up to like & getrecommendations! Published in 2020 at "Multimedia Tools and Applications"
DOI: 10.1007/s11042-020-09010-5
Abstract: Biosensors-based devices are transforming medical diagnosis of diseases and monitoring of patient signals. The development of smart and automated molecular diagnostic tools equipped with biomedical big data analysis, cloud computing and medical artificial intelligence can… read more here.
Keywords: cloud internet; crispr based; futuristic crispr; internet things ... See more keywords
CRISPR-based enrichment strategies for targeted sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2020.107672
Abstract: The ability to easily produce or procure sequencing data has expanded to be within the reach of most clinics and research laboratories, but the complexity of sequence analysis remains a hurdle for many scientists, and… read more here.
Keywords: strategies targeted; enrichment strategies; crispr based; based enrichment ... See more keywords
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
Sign Up to like & getrecommendations! Published in 2017 at "Cell"
DOI: 10.1016/j.cell.2017.04.005
Abstract: The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies… read more here.
Keywords: crispr based; technologies manipulation; manipulation eukaryotic; based technologies ... See more keywords
CRISPR-based tools: Alternative methods for the diagnosis of COVID-19
Sign Up to like & getrecommendations! Published in 2021 at "Clinical Biochemistry"
DOI: 10.1016/j.clinbiochem.2020.12.011
Abstract: The recently emerged severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spread all over the world rapidly and caused a global pandemic. To prevent the virus from spreading to more individuals, it is of great importance to… read more here.
Keywords: detection; crispr based; diagnosis; based tools ... See more keywords
CRISPR-based metabolic engineering in non-model microorganisms.
Sign Up to like & getrecommendations! Published in 2022 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2022.102698
Abstract: Non-model microorganisms possess unique and versatile metabolic characteristics, offering great opportunities as cell factories for biosynthesis of target products. However, lack of efficient genetic tools for pathway engineering represents a big challenge to unlock the… read more here.
Keywords: non model; crispr based; model microorganisms; engineering ... See more keywords
CRISPR-Based Directed Evolution for Crop Improvement.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2019.08.001
Abstract: Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target… read more here.
Keywords: crispr based; crop improvement; directed evolution; evolution ... See more keywords