CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
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DOI: 10.1002/advs.201902312
Abstract: Abstract Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems, which make the targeted modification of… read more here.
Keywords: genome editing; target effects; crispr cas; target ... See more keywords
CRISPR‐Enhanced Hydrogel Microparticles for Multiplexed Detection of Nucleic Acids
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DOI: 10.1002/advs.202206872
Abstract: CRISPR/Cas systems offer a powerful sensing mechanism to transduce sequence‐specific information into amplified analytical signals. However, performing multiplexed CRISPR/Cas assays remains challenging and often requires complex approaches for multiplexed assays. Here, a hydrogel‐based CRISPR/Cas12 system… read more here.
Keywords: crispr; enhanced hydrogel; crispr enhanced; detection ... See more keywords
Repurposing CRISPR/Cas to Discover SARS-CoV-2 Detecting and Neutralizing Aptamers.
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DOI: 10.1002/advs.202300656
Abstract: RNA aptamers provide useful biological probes and therapeutic agents. New methodologies to screen RNA aptamers will be valuable by complementing the traditional Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Meanwhile, repurposing clustered regularly interspaced… read more here.
Keywords: repurposing crispr; crispr cas; rna aptamers; sars cov ... See more keywords
Potential of the CRISPR‐Cas system for improved parasite diagnosis
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DOI: 10.1002/bies.202100286
Abstract: CRISPR‐Cas technology accelerates development of fast, accurate, and portable diagnostic tools, typified by recent applications in COVID‐19 diagnosis. Parasitic helminths cause devastating diseases afflicting 1.5 billion people globally, representing a significant public health and economic… read more here.
Keywords: system; crispr; diagnosis; crispr cas ... See more keywords
Breakthrough in CRISPR/Cas system: Current and future directions and challenges.
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DOI: 10.1002/biot.202200642
Abstract: Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists to develop desirable varieties. This technology involves inducing site-specific DNA perturbations that can be repaired… read more here.
Keywords: genome editing; technology; crispr cas; crispr ... See more keywords
Recognizing CRISPR as the New Age Disease-Modifying Drug: Strategies to Bioengineer CRISPR/Cas for Direct in vivo Delivery.
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DOI: 10.1002/biot.202300077
Abstract: CRISPR has established itself as a frontier technology in genetic engineering. Researchers have successfully used the CRISPR/Cas system as precise gene editing tools and have further expanded their scope beyond both imaging and diagnostic applications.… read more here.
Keywords: cas; crispr cas; gene; vivo delivery ... See more keywords
Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum
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DOI: 10.1002/bit.27753
Abstract: Clostridium butyricum has been widely used as a probiotic for humans and food animals. However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly understood, largely due to the lack of… read more here.
Keywords: genome editing; butyricum; crispr; crispr cas ... See more keywords
CRISPR/Cas‐mediated Fubp1 silencing disrupts circadian oscillation of Per1 protein via downregulating Syncrip expression
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DOI: 10.1002/cbin.11242
Abstract: Most living organisms have physiological and behavioral circadian rhythms controlled by molecular clocks. In mammals, several core clock genes show self‐perpetuating oscillation profiles of their messenger RNAs (mRNAs) and proteins through an auto‐regulatory transcription–translation feedback… read more here.
Keywords: per1 protein; cas mediated; crispr cas; oscillation ... See more keywords
Nanoparticles‐Mediated CRISPR/Cas Gene Editing Delivery System
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DOI: 10.1002/cmdc.202100777
Abstract: CRISPR/Cas system has become one of the most powerful techologies in biomedical research, and has showed great potentials in the gene related diseases. However, efficient delivery systems of CRISPR/Cas to target cells remains challenging. In… read more here.
Keywords: system; nanoparticles mediated; crispr cas; gene ... See more keywords
Current progress in CRISPR‐based diagnostic platforms
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DOI: 10.1002/jcb.27690
Abstract: The CRISPR‐Cas system is a key technology for genome editing and regulation in a wide range of organisms and cell types. Recently, CRISPR‐Cas–based diagnostic platform has shown idealistic properties for pathogen detection. Integrating the CRISPR‐Cas… read more here.
Keywords: progress crispr; crispr; current progress; crispr based ... See more keywords
A comprehensive overview of CRISPR/Cas 9 technology and application thereof in drug discovery
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DOI: 10.1002/jcb.30329
Abstract: Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)‐Cas technology possesses revolutionary potential to positively affect various domains of drug discovery. It has initiated a rise in the area of genetic engineering and its advantages range from… read more here.
Keywords: drug discovery; technology; crispr; crispr cas ... See more keywords