CRISPR–Cas12‐based field‐deployable system for rapid detection of synthetic DNA sequence of the monkeypox virus genome
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28385
Abstract: The global outbreak of the monkeypox virus (MPXV) highlights the need for rapid and cost‐effective MPXV detection tools to effectively monitor and control the monkeypox disease. Herein, we demonstrated a portable CRISPR–Cas‐based system for naked‐eye… read more here.
Keywords: crispr cas12; system; detection; monkeypox virus ... See more keywords
Improved Strategies for CRISPR-Cas12-based Nucleic Acids Detection
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Analysis and Testing"
DOI: 10.1007/s41664-022-00212-4
Abstract: The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology. Thus, it is urgent to develop a more simple and efficient nucleic acid detection technology. CRISPR-Cas12 has signal amplification ability, high sensitivity… read more here.
Keywords: based nucleic; detection; cas12 based; nucleic acid ... See more keywords
G-Quadruplex DNAzyme-Substrated CRISPR/Cas12 Assay for Label-Free Detection of Single-Celled Parasitic Infection.
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DOI: 10.1021/acssensors.2c01104
Abstract: Early diagnosis of parasitic diseases can dramatically alleviate medical, economic, and social burdens. Herein, we report a sensitive and label-free assay for diagnosing single-celled parasitic infections using G-quadruplex (G4) DNAzyme as a reporter for CRISPR/Cas12.… read more here.
Keywords: label free; celled parasitic; crispr cas12; detection ... See more keywords
CS-Cells: A CRISPR-Cas12 DNA Device to Generate Chromosome-Shredded Cells for Efficient and Safe Molecular Biomanufacturing.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00516
Abstract: Synthetic biology holds great promise for translating ideas into products to address the grand challenges facing humanity. Molecular biomanufacturing is an emerging technology that facilitates the production of key products of value, including therapeutics and… read more here.
Keywords: molecular biomanufacturing; dna device; crispr cas12; biology ... See more keywords