CRISPR/Cas12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Journal"
DOI: 10.1002/biot.202000308
Abstract: Most biopharmaceuticals produced today are generated using Chinese hamster ovary (CHO) cells, therefore significant attention is focused on methods to improve CHO cell productivity and product quality. The discovery of gene‐editing tools, such as CRISPR/Cas9,… read more here.
Keywords: crispr cas12a; cho; cell; cell line ... See more keywords
Cascade DNA Circuits Mediated CRISPR-Cas12a Fluorescent Aptasensor based on Multifunctional Fe3 O4 @hollow-TiO2 @MoS2 Nanochains for Tetracycline Determination.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202206105
Abstract: Herein, for the first time, the CRISPR-Cas12a system is combined with aptamer, cascaded dynamic DNA network circuits, and Fe3 O4 @hollow-TiO2 @MoS2 nanochains (Fe3 O4 @h-TiO2 @MoS2 NCs) to construct an efficient sensing platform for… read more here.
Keywords: mos2; crispr cas12a; tio2 mos2; dna ... See more keywords
Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_7
Abstract: The oleaginous yeast Yarrowia lipolytica has emerged as an industrially relevant chassis to produce various valuable chemicals. Metabolic engineering of Y. lipolytica relies on the availability of genetic engineering tools. Existing engineering strategies for this yeast… read more here.
Keywords: genome editing; crispr cas12a; efficient genome; lipolytica ... See more keywords
Target recognition triggered CRISPR-Cas12a assisted allosteric scaffold for sensitively analyzing bacterial infection after dental implantation.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2022.114666
Abstract: Accurate identification and sensitive quantification of infected bacteria after dental implant both play crucial roles in early-diagnosis of bacterial infection and guiding medicine applications. Herein, we propose a sensitive and accurate bacteria detection method based… read more here.
Keywords: bacterial infection; assisted allosteric; crispr cas12a; allosteric scaffold ... See more keywords
CRISPR-Cas12a-based efficient electrochemiluminescence biosensor for ATP detection.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2021.339180
Abstract: CRISPR-Cas12a system exhibits tremendous potential in accurate recognition and quantitation of nucleic acids and non-nucleic-acid targets thanks to the discovery of its cleavage capability toward single-stranded DNA (ssDNA). In this study, we developed an efficient… read more here.
Keywords: crispr cas12a; detection; crispr; cas12a ... See more keywords
Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp
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DOI: 10.1016/j.aquaculture.2019.734340
Abstract: Abstract White spot disease (WSD), caused by white spot syndrome virus (WSSV), is among the most severe diseases of cultivated shrimp. Here, the CRISPR-Cas12a system coupled with nucleic acid amplification was optimized for the detection… read more here.
Keywords: amplification; cas12a; crispr cas12a; white spot ... See more keywords
An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip
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DOI: 10.1016/j.bioactmat.2021.05.005
Abstract: CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. However, cleavage of the amplicons and primers by the cis- and trans-activity of Cas12a hinders the attempts… read more here.
Keywords: crispr cas12a; detection; nucleic acid; strip ... See more keywords
Enzymatic Recombinase Amplification Coupled with CRISPR-Cas12a for Ultrasensitive, Rapid, and Specific Porcine circovirus 3 Detection.
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DOI: 10.1016/j.mcp.2021.101763
Abstract: Porcine circovirus type 3 (PCV3) is a disease associated with porcine dermatitis and nephrotic syndrome (PDNS) that has caused significant economic losses to swine herds since its discovery in 2016. To develop a simple, on-site,… read more here.
Keywords: crispr cas12a; cas12a; porcine circovirus; enzymatic recombinase ... See more keywords
Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections.
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DOI: 10.1016/j.mimet.2021.106167
Abstract: Early-diagnosis and treatments of skin and soft tissue infections remain a huge challenge due to the difficulties in the detection of trace amounts of bacteria. We develop here a novel method through CRISPR-Cas12a based recycling… read more here.
Keywords: crispr cas12a; skin soft; soft tissue; cas12a ... See more keywords
A strategy combining 3D-DNA Walker and CRISPR-Cas12a trans-cleavage activity applied to MXene based electrochemiluminescent sensor for SARS-CoV-2 RdRp gene detection
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DOI: 10.1016/j.talanta.2021.122868
Abstract: Early diagnosis and timely management of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are the keys to preventing the spread of the epidemic and controlling new infection clues. Therefore, strengthening the surveillance of the epidemic… read more here.
Keywords: sars cov; rdrp gene; crispr cas12a; dna ... See more keywords
Dipstick-based rapid nucleic acids purification and CRISPR/Cas12a-mediated isothermal amplification for visual detection of African swine fever virus.
Sign Up to like & getrecommendations! Published in 2022 at "Talanta"
DOI: 10.1016/j.talanta.2022.123294
Abstract: African swine fever virus (ASFV) can cause highly contagious and fatal disease among domestic pigs, resulting in considerable economic losses for swine breeders. There is a strong demand for accurate, rapid, and simple detection methods… read more here.
Keywords: purification; crispr cas12a; nucleic acids; detection ... See more keywords