A one-step platform for screening high-efficient and minimal off-target CRISPR/Cas13 crRNAs to eradicate SARS-CoV-2 virus for treatment of COVID-19 patients
Sign Up to like & getrecommendations! Published in 2022 at "Medical Hypotheses"
DOI: 10.1016/j.mehy.2021.110754
Abstract: Coronavirus disease 2019 (COVID-19) is a new respiratory illness caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and now spreads globally. Currently, therapeutics and effective treatment options remain scarce and there is no… read more here.
Keywords: high efficient; sars cov; covid; crispr cas13 ... See more keywords
Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.12.018
Abstract: Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 approach to image colocalized or repeat-containing RNAs in real time, as well… read more here.
Keywords: crispr cas13; image; time; real time ... See more keywords
CRISPR-Cas13-Mediated Knockdown of Regulator of G-Protein Signaling 8 (RGS8) Does Not Affect Purkinje Cell Dendritic Development
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.854273
Abstract: CRISPR-Cas13 technology is rapidly evolving as it is a very specific tool for RNA editing and interference. Since there are no significant off-target effects via the Cas13-mediated method, it is a promising tool for studying… read more here.
Keywords: regulator protein; cas13; crispr cas13; purkinje ... See more keywords
CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1011399
Abstract: Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA… read more here.
Keywords: cas13 new; detection pathogenic; pathogenic microorganisms; crispr cas13 ... See more keywords
CRISPR-Cas13 in malaria parasite: Diagnosis and prospective gene function identification
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1076947
Abstract: Malaria caused by Plasmodium is still a serious public health problem. Genomic editing is essential to understand parasite biology, elucidate mechanical pathways, uncover gene functions, identify novel therapeutic targets, and develop clinical diagnostic tools. Recent… read more here.
Keywords: crispr cas13; plasmodium; gene; cas13 malaria ... See more keywords
Engineering CRISPR/Cas13 System against RNA Viruses: From Diagnostics to Therapeutics
Sign Up to like & getrecommendations! Published in 2022 at "Bioengineering"
DOI: 10.3390/bioengineering9070291
Abstract: Over the past decades, RNA viruses have been threatened people’s health and led to global health emergencies. Significant progress has been made in diagnostic methods and antiviral therapeutics for combating RNA viruses. ELISA and RT-qPCR… read more here.
Keywords: crispr cas13; rna viruses; cas13 system;
CRISPR/Cas13-Based Platforms for a Potential Next-Generation Diagnosis of Colorectal Cancer through Exosomes Micro-RNA Detection: A Review
Sign Up to like & getrecommendations! Published in 2021 at "Cancers"
DOI: 10.3390/cancers13184640
Abstract: Simple Summary Colorectal cancer is one of the most prevalent cancers, whereas a significant number of cases are diagnosed in late cancer stages, and survival rates drop dramatically. Micro-RNAs (miRNAs) from cancer-derived exosomes have shown… read more here.
Keywords: crispr cas13; based platforms; diagnosis; cas13 based ... See more keywords
Insights Gained from RNA Editing Targeted by the CRISPR-Cas13 Family
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms231911400
Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, especially type II (Cas9) systems, have been widely developed for DNA targeting and formed a set of mature precision gene-editing systems. However, the basic research… read more here.
Keywords: rna editing; crispr cas13; cas13 family; family ... See more keywords
The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons
Sign Up to like & getrecommendations! Published in 2021 at "Toxins"
DOI: 10.3390/toxins13040262
Abstract: The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is… read more here.
Keywords: cas13; bacterial enzyme; cortical neurons; crispr cas13 ... See more keywords