Collateral Effects: The CRISPR‐Cas13a Gene‐Editing System Induces Collateral Cleavage of RNA in Glioma Cells (Adv. Sci. 20/2019)
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201970124
Abstract: In article number https://doi.org/10.1002/advs.201901299, Chunsheng Kang and co‐workers demonstrate the collateral effect of the CRISPR‐Cas13a system in glioma cells. EGFRvIII is a unique EGFR mutant subtype in glioma, and the CRISPR‐Cas13a system induces death in… read more here.
Keywords: system; crispr cas13a; collateral effects; glioma cells ... See more keywords
One‐Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point‐of‐Care Testing
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Medical Virology"
DOI: 10.1002/jmv.70115
Abstract: Hepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, and easy‐to‐use detection. This study aimed to develop a One‐Pot assay using… read more here.
Keywords: one pot; hev; crispr cas13a; pot assay ... See more keywords
In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.
Sign Up to like & getrecommendations! Published in 2020 at "Antiviral research"
DOI: 10.1016/j.antiviral.2020.104794
Abstract: Sustained infection of high-risk human papillomavirus (HR-HPVs), especially HPV16 and HPV18,is a major cause of cervical cancer. E6 and E7 oncoproteins, encoded by the HPV genome, are critical for transformation and maintenance of malignant phenotypes… read more here.
Keywords: crispr cas13a; cervical cancer; cas13a system;
A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment.
Sign Up to like & getrecommendations! Published in 2018 at "Cancer letters"
DOI: 10.1016/j.canlet.2018.05.042
Abstract: Mutant KRAS is a known driver oncogene in pancreatic cancer. However, this protein remains an "undruggable" therapeutic target. Inhibiting mutated KRAS expression at the mRNA level is a potentially effective strategy. Recently, a novel CRISPR-Cas… read more here.
Keywords: crispr cas13a; mutant kras; cas13a; cas13a system ... See more keywords
High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03708
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) (CRISPR/Cas) system innovates a next-generation biosensor due to its high-fidelity, programmability, and efficient signal amplification ability. Developing a CRISPR/Cas-based visual detection system could contribute… read more here.
Keywords: high fidelity; crispr cas13a; detection; trans cleavage ... See more keywords
Thermophoretic Aggregation Imaging of Tumor-Derived Exosomes by CRISPR-Cas13a-Based Nanoprobes.
Sign Up to like & getrecommendations! Published in 2024 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c04646
Abstract: The inherent heterogeneity of tumor-derived exosomes holds great promise for enhancing the precision of cancer diagnostics. MicroRNAs (miRNAs) encapsulated in tumor-associated exosomes have emerged as valuable biomarkers for the early detection of cancers. Nevertheless, the… read more here.
Keywords: crispr cas13a; cancer; tumor derived; thermophoretic aggregation ... See more keywords
CRISPR/Cas13a-Responsive and RNA-Bridged DNA Hydrogel Capillary Sensor for Point-of-Care Detection of RNA
Sign Up to like & getrecommendations! Published in 2024 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.4c02087
Abstract: Disease diagnostics and surveillance increasingly highlight the importance of portable, cost-effective, and sensitive point-of-care (POC) detection of nucleic acids. Here, we report a CRISPR/Cas13a-responsive and RNA-bridged DNA hydrogel capillary sensor for the direct and visual… read more here.
Keywords: detection; crispr cas13a; hydrogel; rna ... See more keywords
Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.2c00200
Abstract: In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to the duck-raising industry. To succeed in preventing and controlling infections, extant techniques… read more here.
Keywords: system; rpa based; crispr cas13a; detection ... See more keywords
Advanced Cancer Liquid Biopsy Platform for miRNA Detection in Extracellular Vesicles Using CRISPR/Cas13a and Gold Nanoarrays
Sign Up to like & getrecommendations! Published in 2025 at "ACS Nano"
DOI: 10.1021/acsnano.5c06940
Abstract: Liquid biopsy is a transformative, noninvasive tool for cancer diagnosis and monitoring, with the potential to revolutionize personalized medicine. In this study, we introduce an advanced liquid biopsy platform for highly sensitive and selective detection… read more here.
Keywords: detection; crispr cas13a; cancer; liquid biopsy ... See more keywords
Rapid and Unamplified Detection of SARS-CoV-2 RNA via CRISPR-Cas13a-Modified Solution-Gated Graphene Transistors
Sign Up to like & getrecommendations! Published in 2022 at "ACS Sensors"
DOI: 10.1021/acssensors.2c01990
Abstract: The disease caused by severe acute respiratory syndrome coronavirus, SARS-CoV-2, is termed COVID-19. Even though COVID-19 has been out for more than two years, it is still causing a global pandemic. Due to the limitations… read more here.
Keywords: crispr cas13a; detection; sars cov; solution gated ... See more keywords
Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS.
Sign Up to like & getrecommendations! Published in 2025 at "ACS sensors"
DOI: 10.1021/acssensors.5c02084
Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) has been the gold standard for SARS-CoV-2 detection during the COVID-19 pandemic. However, its requirement for RNA-to-DNA conversion, reliance on centralized laboratory infrastructure, and lengthy turnaround times have limited its… read more here.
Keywords: sars cov; crispr cas13a; dual pathway; cas13a mediated ... See more keywords