Collateral Effects: The CRISPR‐Cas13a Gene‐Editing System Induces Collateral Cleavage of RNA in Glioma Cells (Adv. Sci. 20/2019)
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201970124
Abstract: In article number https://doi.org/10.1002/advs.201901299, Chunsheng Kang and co‐workers demonstrate the collateral effect of the CRISPR‐Cas13a system in glioma cells. EGFRvIII is a unique EGFR mutant subtype in glioma, and the CRISPR‐Cas13a system induces death in… read more here.
Keywords: system; crispr cas13a; collateral effects; glioma cells ... See more keywords
In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.
Sign Up to like & getrecommendations! Published in 2020 at "Antiviral research"
DOI: 10.1016/j.antiviral.2020.104794
Abstract: Sustained infection of high-risk human papillomavirus (HR-HPVs), especially HPV16 and HPV18,is a major cause of cervical cancer. E6 and E7 oncoproteins, encoded by the HPV genome, are critical for transformation and maintenance of malignant phenotypes… read more here.
Keywords: crispr cas13a; cervical cancer; cas13a system;
A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment.
Sign Up to like & getrecommendations! Published in 2018 at "Cancer letters"
DOI: 10.1016/j.canlet.2018.05.042
Abstract: Mutant KRAS is a known driver oncogene in pancreatic cancer. However, this protein remains an "undruggable" therapeutic target. Inhibiting mutated KRAS expression at the mRNA level is a potentially effective strategy. Recently, a novel CRISPR-Cas… read more here.
Keywords: crispr cas13a; mutant kras; cas13a; cas13a system ... See more keywords
High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03708
Abstract: The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) (CRISPR/Cas) system innovates a next-generation biosensor due to its high-fidelity, programmability, and efficient signal amplification ability. Developing a CRISPR/Cas-based visual detection system could contribute… read more here.
Keywords: high fidelity; crispr cas13a; detection; trans cleavage ... See more keywords
Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.2c00200
Abstract: In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to the duck-raising industry. To succeed in preventing and controlling infections, extant techniques… read more here.
Keywords: system; rpa based; crispr cas13a; detection ... See more keywords
Rapid and Unamplified Detection of SARS-CoV-2 RNA via CRISPR-Cas13a-Modified Solution-Gated Graphene Transistors
Sign Up to like & getrecommendations! Published in 2022 at "ACS Sensors"
DOI: 10.1021/acssensors.2c01990
Abstract: The disease caused by severe acute respiratory syndrome coronavirus, SARS-CoV-2, is termed COVID-19. Even though COVID-19 has been out for more than two years, it is still causing a global pandemic. Due to the limitations… read more here.
Keywords: crispr cas13a; detection; sars cov; solution gated ... See more keywords
Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-019-14135-9
Abstract: The ability to detect low numbers of microbial cells in food and clinical samples is highly valuable but remains a challenge. Here we present a detection system (called ‘APC-Cas’) that can detect very low numbers… read more here.
Keywords: detection; crispr cas13a; low numbers; bacterial pathogen ... See more keywords
Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16731-6
Abstract: The emergence of antimicrobial-resistant bacteria is an increasingly serious threat to global health, necessitating the development of innovative antimicrobials. Here we report the development of a series of CRISPR-Cas13a-based antibacterial nucleocapsids, termed CapsidCas13a(s), capable of… read more here.
Keywords: crispr cas13a; cas13a based; development; target ... See more keywords
Rapid detection of insect pest with CRISPR‐Cas13a‐Based Lateral Flow Strip: Using Locusta migratoria manilensis
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Applied Entomology"
DOI: 10.1111/jen.13121
Abstract: The Clustered Regularly Interspaced Short Palindromic Repeats‐associated (CRISPR‐Cas) system has been used as a powerful diagnostic tool because of its trans‐cleavage ability to accurately recognize and cleave specific nucleic acid targets. However, its application in… read more here.
Keywords: system; cas13a; migratoria manilensis; crispr cas13a ... See more keywords
Hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abn7382
Abstract: CRISPR-Cas13a holds enormous potential for developing precise RNA editing. However, spatial manipulation of CRISPR-Cas13a activity remains a daunting challenge for elaborately regulating localized RNase function. Here, we designed hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons (RNCOs-D), featuring… read more here.
Keywords: cas13a; therapy; crispr cas13a; hierarchical self ... See more keywords
Nucleic acid detection with CRISPR-Cas13a/C2c2
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.aam9321
Abstract: Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a… read more here.
Keywords: detection; crispr; cas13a; dna ... See more keywords