Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs
Sign Up to like & getrecommendations! Published in 2021 at "Genome Biology"
DOI: 10.1186/s13059-021-02263-9
Abstract: Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool… read more here.
Keywords: functional circrnas; optimized rna; rna targeting; crispr cas13d ... See more keywords
Shotgun knockdown of RNA by CRISPR-Cas13d in fission yeast.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cell science"
DOI: 10.1242/jcs.260769
Abstract: The CRISPR-Cas13d system has a single small effector protein that targets RNA and does not require the presence of a protospacer flanking site in the targeted transcript. These features make CRISPR-Cas13d an attractive system for… read more here.
Keywords: cas13d system; crispr cas13d; fission yeast; cas13d ... See more keywords
Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-β/Smad Signaling
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2020.616768
Abstract: A subset of long non-coding RNAs (lncRNAs), categorized as miRNA-host gene lncRNAs (lnc-miRHGs), is processed to produce miRNAs and involved in cancer progression. This work aimed to investigate the influences and the molecular mechanisms of… read more here.
Keywords: bca; mir497hg; crispr cas13d; cancer ... See more keywords
Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2
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DOI: 10.3390/v15030686
Abstract: The current SARS-CoV-2 pandemic forms a major global health burden. Although protective vaccines are available, concerns remain as new virus variants continue to appear. CRISPR-based gene-editing approaches offer an attractive therapeutic strategy as the CRISPR-RNA… read more here.
Keywords: antiviral strategy; crispr cas13d; strategy; sars cov ... See more keywords