Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202102365
Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.
Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
Sign Up to like & getrecommendations! Published in 2019 at "Advanced materials"
DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage
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DOI: 10.1002/bies.202000047
Abstract: Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing… read more here.
Keywords: crispr cas9; cas9 target; cas9 editing; target ... See more keywords
Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100434
Abstract: Alternative cancer models that are close to humans are required to create more valuable preclinical results during oncology studies. Here, a new onco‐pig model via developing a CRISPR‐Cas9‐based Conditional Polycistronic gene expression Cassette (CRI‐CPC) system… read more here.
Keywords: crispr cas9; system; cri cpc; cas9 based ... See more keywords
CRISPR/Cas9-mediated knockout of a prolyl-4-hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology journal"
DOI: 10.1002/biot.202100698
Abstract: The properties of host plants used for molecular farming can be modified by CRISPR/Cas9 genome editing to improve the quality and yield of recombinant proteins. However, it is often necessary to target multiple genes simultaneously,… read more here.
Keywords: nicotiana benthamiana; prolyl hydroxylase; crispr cas9; plant ... See more keywords
Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR‐Cas9 Activation
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800266
Abstract: Silent biosynthetic gene clusters represent a potentially rich source of new bioactive compounds. We report the discovery, characterization, and biosynthesis of a novel doubly glycosylated 24‐membered polyene macrolactam from a silent biosynthetic gene cluster in… read more here.
Keywords: crispr cas9; gene cluster; streptomyces roseosporus; activation ... See more keywords
Genome‐Scale CRISPR/Cas9 Screening Reveals Squalene Epoxidase as a Susceptibility Factor for Cytotoxicity of Malformin A1
Sign Up to like & getrecommendations! Published in 2019 at "Chembiochem"
DOI: 10.1002/cbic.201800769
Abstract: Malformin A1 (MA1) is a fungus‐produced cyclic pentapeptide. MA1 exhibits teratogenicity to plants, fibrinolysis‐enhancing activity, and cytotoxicity to mammalian cells. To clarify the cytotoxic mechanism of MA1, we screened for the genes involved in the cytotoxicity… read more here.
Keywords: crispr cas9; ma1; cytotoxicity; squalene epoxidase ... See more keywords
Genome Editing in Mice Using CRISPR/Cas9 Technology
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DOI: 10.1002/cpcb.57
Abstract: CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA‐guided nuclease Cas9 to a designated genomic site… read more here.
Keywords: genome editing; crispr cas9; cas9 technology; cas9 ... See more keywords
Efficient CRISPR/Cas9‐Mediated Mutagenesis in Primary Murine T Lymphocytes
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DOI: 10.1002/cpim.62
Abstract: The ability to alter gene expression directly in T lymphocytes has provided a powerful tool for understanding T cell biology, signaling, and function. Manipulation of T cell clones and primary T cells has been accomplished… read more here.
Keywords: mediated mutagenesis; mutagenesis primary; crispr cas9; cas9 mediated ... See more keywords
Chemical Modification and Transformation Strategies of Guide RNAs in CRISPR-Cas9 Gene Editing Systems.
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DOI: 10.1002/cplu.202000785
Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated protein 9) is a most powerful tool and has been widely used in gene editing and gene regulation since its discovery. However, wild-type CRISPR-Cas9 suffers from… read more here.
Keywords: guide rnas; gene editing; crispr cas9; gene ... See more keywords
Single nucleotide editing without DNA cleavage using CRISPR/Cas9‐deaminase in the sea urchin embryo
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DOI: 10.1002/dvdy.24586
Abstract: Background: A single base pair mutation in the genome can result in many congenital disorders in humans. The recent gene editing approach using CRISPR/Cas9 has rapidly become a powerful tool to replicate or repair such… read more here.
Keywords: crispr cas9; single nucleotide; nucleotide editing; sea urchin ... See more keywords