Remodeling the Neuroimmune Microenvironment in Retinal Ischemia‐Reperfusion Injury via Combined CRISPR/Cas9 Targeting of ACSL4 and Isorhamnetin
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202505041
Abstract: Retinal ischemia‐reperfusion (RIR) injury induces oxidative stress, excitotoxicity, inflammation, and ferroptosis, which interact through complex crosstalk, forming a retinal pathological microenvironment (RPMe) that drives retinal ganglion cell (RGC) death. Central to these processes is the… read more here.
Keywords: neuroimmune microenvironment; retinal ischemia; ischemia reperfusion; crispr cas9 ... See more keywords
Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers
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DOI: 10.1002/adhm.202102365
Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.
Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords
A Galactose-Engineered Dual-Responsive Nanocarrier for ASO/CRISPR-Cas9 Delivery to Inhibit HBV Replication.
Sign Up to like & getrecommendations! Published in 2025 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202502835
Abstract: Complete hepatitis B virus (HBV) cure is hindered primarily by the stable persistence of covalently closed circular DNA (cccDNA). Gene editing approaches to eradicate HBV by targeting cccDNA face challenges and limitations due to suboptimal… read more here.
Keywords: hbv replication; hbv; delivery; crispr cas9 ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
Sign Up to like & getrecommendations! Published in 2019 at "Advanced materials"
DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Magnetic Nanoparticle‐Assisted Non‐Viral CRISPR‐Cas9 for Enhanced Genome Editing to Treat Rett Syndrome
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DOI: 10.1002/advs.202306432
Abstract: The CRISPR‐Cas9 technology has the potential to revolutionize the treatment of various diseases, including Rett syndrome, by enabling the correction of genes or mutations in human patient cells. However, several challenges need to be addressed… read more here.
Keywords: crispr; rett syndrome; genome editing; magnetic nanoparticle ... See more keywords
CRISPR/Cas9 mediated validation of spermatogenesis-related gene, tssk2 as a component of genetic pest management of fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae).
Sign Up to like & getrecommendations! Published in 2024 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.22121
Abstract: Invasive insect pests, currently, pose a serious economic threat to several staple crops all over the world, one such being the fall armyworm, Spodoptera frugiperda. It was first observed in Africa since 2016, outside of… read more here.
Keywords: cas9; spodoptera frugiperda; armyworm spodoptera; management ... See more keywords
Cas9 Cuts and Consequences; Detecting, Predicting, and Mitigating CRISPR/Cas9 On‐ and Off‐Target Damage
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DOI: 10.1002/bies.202000047
Abstract: Large deletions and genomic re‐arrangements are increasingly recognized as common products of double‐strand break repair at Clustered Regularly Interspaced, Short Palindromic Repeats ‐ CRISPR associated protein 9 (CRISPR/Cas9) on‐target sites. Together with well‐known off‐target editing… read more here.
Keywords: crispr cas9; cas9 target; cas9 editing; target ... See more keywords
Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100434
Abstract: Alternative cancer models that are close to humans are required to create more valuable preclinical results during oncology studies. Here, a new onco‐pig model via developing a CRISPR‐Cas9‐based Conditional Polycistronic gene expression Cassette (CRI‐CPC) system… read more here.
Keywords: crispr cas9; system; cri cpc; cas9 based ... See more keywords
CRISPR/Cas9-mediated knockout of a prolyl-4-hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection.
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DOI: 10.1002/biot.202100698
Abstract: The properties of host plants used for molecular farming can be modified by CRISPR/Cas9 genome editing to improve the quality and yield of recombinant proteins. However, it is often necessary to target multiple genes simultaneously,… read more here.
Keywords: nicotiana benthamiana; prolyl hydroxylase; crispr cas9; plant ... See more keywords
Overexpression of Klotho gene using CRISPR/Cas9 induces apoptosis and inhibits cell motility in the human colorectal cancer cells
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DOI: 10.1002/biot.202300496
Abstract: Despite advances in early detection and treatment, colorectal cancer remains one of the leading causes of cancer‐related deaths. The klotho (KL) gene plays a critical role in the development and progression of colorectal cancer. This… read more here.
Keywords: colorectal cancer; cancer; cancer cells; gene ... See more keywords
Resolving the Trade‐Off Between Toxicity and Efficiency of CRISPR‐Cas9 System for Genome Editing Within Escherichia coli
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DOI: 10.1002/biot.70010
Abstract: Efficient gene editing of Escherichia coli BL21 (DE3) holds significant practical value as a host for heterologous protein expression. Recently reported CRISPR‐Cas9 editing systems for this strain exhibit a trade‐off between efficiency and toxicity. In… read more here.
Keywords: trade; system; efficiency; cas9 ... See more keywords