Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation.
Sign Up to like & getrecommendations! Published in 2017 at "Stem cell research"
DOI: 10.1016/j.scr.2017.07.007
Abstract: Krueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15… read more here.
Keywords: using paired; paired crispr; line using; klf15 ... See more keywords
Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-019-1537-1
Abstract: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in multiplex genome editing technologies. However, simultaneously modulating multiple genes on the… read more here.
Keywords: genome editing; system; efficiency; crispr cas9n ... See more keywords