CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800195
Abstract: In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of… read more here.
Keywords: fragment deletion; crispr cpf1; saccharomyces cerevisiae; large fragment ... See more keywords
Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems
Sign Up to like & getrecommendations! Published in 2018 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-018-2810-3
Abstract: CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only in human cells and… read more here.
Keywords: genome editing; system; mammalian systems; crispr cpf1 ... See more keywords
Multiplex genome editing in Ashbya gossypii using CRISPR-Cpf1.
Sign Up to like & getrecommendations! Published in 2020 at "New biotechnology"
DOI: 10.1016/j.nbt.2020.02.002
Abstract: CRISPR/Cas technologies constitute essential tools for rapid genome engineering of many organisms, including fungi. The CRISPR/Cas9 system adapted for the industrial fungus Ashbya gossypii enables efficient genome editing for the introduction of deletions, insertions and… read more here.
Keywords: genome editing; crispr cpf1; system; ashbya gossypii ... See more keywords
Combining CRISPR-Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00041
Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR)-based gene-editing technology has been widely used in various microorganisms due to its advantages of low cost, high efficiency, easy operation, and multiple functions. In this study, an efficient… read more here.
Keywords: system; crispr cpf1; gene; escherichia coli ... See more keywords
CRISPR/Cpf1-Mediated Multiplex and Large-Fragment Gene Editing in Staphylococcus aureus.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00248
Abstract: Staphylococcus aureus is a major human pathogen that causes a variety of infections, including life-threatening diseases. Research on S. aureus is constrained by complex and limited genetic manipulation methods. Here, we report a CRISPR/Cpf1-mediated system,… read more here.
Keywords: cpf1 mediated; system; crispr; gene editing ... See more keywords
Multiplexed CRISPR-Cpf1-Mediated Genome Editing in Clostridium difficile toward the Understanding of Pathogenesis of C. difficile Infection.
Sign Up to like & getrecommendations! Published in 2018 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00087
Abstract: Clostridium difficile is often the primary cause of nosocomial diarrhea, leading to thousands of deaths annually worldwide. The availability of an efficient genome editing tool for C. difficile is essential to understanding its pathogenic mechanism… read more here.
Keywords: genome editing; clostridium difficile; difficile; crispr cpf1 ... See more keywords
Deep learning improves prediction of CRISPR–Cpf1 guide RNA activity
Sign Up to like & getrecommendations! Published in 2018 at "Nature Biotechnology"
DOI: 10.1038/nbt.4061
Abstract: We present two algorithms to predict the activity of AsCpf1 guide RNAs. Indel frequencies for 15,000 target sequences were used in a deep-learning framework based on a convolutional neural network to train Seq-deepCpf1. We then… read more here.
Keywords: prediction crispr; learning improves; crispr cpf1; deep learning ... See more keywords
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms15179
Abstract: Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into… read more here.
Keywords: genome editing; cpf1 assisted; crispr cpf1; cpf1 ... See more keywords
Genome editing: The efficient tool CRISPR–Cpf1
Sign Up to like & getrecommendations! Published in 2017 at "Nature Plants"
DOI: 10.1038/nplants.2017.28
Abstract: The novel features of the CRISPR–Cpf1 RNA-guided endonuclease system facilitate precise and efficient genome engineering. Application of CRISPR–Cpf1 in plants shows promise for robust gene editing and regulation, opening exciting possibilities for targeted trait improvement… read more here.
Keywords: efficient tool; genome editing; crispr; crispr cpf1 ... See more keywords
Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03760-5
Abstract: Human pluripotent stem cells (hPSCs) have potential applications in biological studies and regenerative medicine. However, precise genome editing in hPSCs remains time-consuming and labor-intensive. Here we demonstrate that the recently identified CRISPR-Cpf1 can be used… read more here.
Keywords: genome editing; human pluripotent; small molecules; crispr cpf1 ... See more keywords
CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity
Sign Up to like & getrecommendations! Published in 2019 at "Bioinformatics"
DOI: 10.1093/bioinformatics/bty1061
Abstract: Motivation The CRISPR-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different gRNA sequences. Results In this study, we reanalyzed the published CRISPR-Cpf1 gRNAs data and… read more here.
Keywords: target efficiency; crispr cpf1; crispr;