LAUSR

About LAUSR
Donate
Contact Us

Follow us:

You are not signed in:

Sign Up!


   Articles with "crispr dcas9" as a keyword



Photo from wikipedia

Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo

Sign Up to like & get
recommendations! Published in 2019 at "Advanced Science"

DOI: 10.1002/advs.201801423

Abstract: Abstract CRISPR/dCas9 systems can precisely control endogenous gene expression without interrupting host genomic sequence and have provided a novel and feasible strategy for the treatment of cancers at the transcriptional level. However, development of CRISPR/dCas9‐based… read more here.

Keywords: crispr; vivo; delivery; crispr dcas9 ... See more keywords

Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors.

Sign Up to like & get
recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7315-6_12

Abstract: Besides genome editing, the CRISPR-Cas9-based platform provides a new way of engineering artificial transcription factors (ATFs). Multiplex of guide RNA (gRNA) expression cassettes holds a great promise for many useful applications of CRISPR-Cas9. In this… read more here.

Keywords: transcription factors; artificial transcription; crispr; crispr dcas9 ... See more keywords

CRISPR-dCas9-Mediated PTEN Activation via Tumor Cell Membrane-Coated Nanoplatform Enhances Sensitivity to Tyrosine Kinase Inhibitors in Nonsmall Cell Lung Cancer.

Sign Up to like & get
recommendations! Published in 2025 at "ACS applied materials & interfaces"

DOI: 10.1021/acsami.4c21740

Abstract: EGFR tyrosine kinase inhibitors (EGFR-TKIs) have garnered substantial clinical success in treating nonsmall cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutations. However, the inevitable emergence of drug resistance, frequently attributed to activation,… read more here.

Keywords: crispr dcas9; lung; lung cancer; activation ... See more keywords

Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation.

Sign Up to like & get
recommendations! Published in 2022 at "ACS chemical biology"

DOI: 10.1021/acschembio.2c00101

Abstract: Inducible CRISPR-dCas9 transcription system has become a powerful tool for transcription regulation and sensing. Here, we develop a new concept of small-molecule-mediated split-aptamer assembly for inducible CRISPR-dCas9 transcription activation, allowing quantitative detection and imaging of… read more here.

Keywords: inducible crispr; dcas9 transcription; transcription; dcas9 ... See more keywords

Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus.

Sign Up to like & get
recommendations! Published in 2023 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.2c00433

Abstract: Fluorescence microscopy imaging of specific chromosomal sites is essential for genome architecture research. To enable visualization of endogenous loci in mammalian cells, programmable DNA-binding proteins such as TAL effectors and CRISPR/dCas9 are commonly utilized. In… read more here.

Keywords: imaging method; replication; replication timing; crispr dcas9 ... See more keywords

The therapeutic implications of all-in-one AAV-delivered epigenome-editing platform in neurodegenerative disorders

Sign Up to like & get
recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-50515-6

Abstract: Safely and efficiently controlling gene expression is a long-standing goal of biomedical research, and CRISPR/Cas system can be harnessed to create powerful tools for epigenetic editing. Adeno-associated-viruses (AAVs) represent the delivery vehicle of choice for… read more here.

Keywords: crispr dcas9; system; aav; dcas9 ... See more keywords

P5387CRISPR/dCas9 Activated Expression of Cardiomyocyte Differentiation Factors in CDCs in Myocardial Infarctions

Sign Up to like & get
recommendations! Published in 2019 at "European Heart Journal"

DOI: 10.1093/eurheartj/ehz746.0347

Abstract: Existing therapies against myocardial infarction (MI) involve disease management by preventing additional damage to the heart muscle. However, new treatment strategies are in greater demand, which deems to focus on restoring cardiac function by replacing… read more here.

Keywords: differentiation factors; cdcs; expression; differentiation ... See more keywords

Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators

Sign Up to like & get
recommendations! Published in 2022 at "Horticulture Research"

DOI: 10.1093/hr/uhab037

Abstract: Abstract Overexpression and knockout (or knockdown) of gene of interest are two commonly used strategies for gene functional study. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene knockout has been applied in most plant… read more here.

Keywords: grape; activation; based transcriptional; gene ... See more keywords

P–567 Re-establishment of fertilization competency of the oocyte via CRISPR/dCas9 epigenome edition technology

Sign Up to like & get
recommendations! Published in 2021 at "Human Reproduction"

DOI: 10.1093/humrep/deab130.566

Abstract: Is it possible to increase the decreased levels of the sperm-oocyte binding protein, Juno, to restore the fertilization capacity of the oocyte, via the use of the CRISPR/dCas9 activation system? JUNO domain (in the oocyte)… read more here.

Keywords: fertilization; system; sperm binding; oocyte ... See more keywords

MiR-ON-CRISPR: a microRNA-activated CRISPR–dCas9 system for precise gene therapy in living cells and mouse models of sepsis

Sign Up to like & get
recommendations! Published in 2025 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkaf1037

Abstract: Abstract The CRISPR–dCas9 technology is a powerful tool for manipulating the expression of target genes in a variety of biomedical applications. Nevertheless, it is imperative that the activity of the CRISPR–dCas9 system be tightly controlled… read more here.

Keywords: mir crispr; system; crispr dcas9; dcas9 system ... See more keywords

Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system

Sign Up to like & get
recommendations! Published in 2019 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkz709

Abstract: Abstract Establishing causal relationship between epigenetic marks and gene transcription requires molecular tools, which can precisely modify specific genomic regions. Here, we present a modular and extensible CRISPR/dCas9-based toolbox for epigenetic editing and direct gene… read more here.

Keywords: dcas9; system; dcas9 based; gene ... See more keywords