Multistage Delivery Nanoparticle Facilitates Efficient CRISPR/dCas9 Activation and Tumor Growth Suppression In Vivo
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Science"
DOI: 10.1002/advs.201801423
Abstract: Abstract CRISPR/dCas9 systems can precisely control endogenous gene expression without interrupting host genomic sequence and have provided a novel and feasible strategy for the treatment of cancers at the transcriptional level. However, development of CRISPR/dCas9‐based… read more here.
Keywords: crispr; vivo; delivery; crispr dcas9 ... See more keywords
Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factors.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7315-6_12
Abstract: Besides genome editing, the CRISPR-Cas9-based platform provides a new way of engineering artificial transcription factors (ATFs). Multiplex of guide RNA (gRNA) expression cassettes holds a great promise for many useful applications of CRISPR-Cas9. In this… read more here.
Keywords: transcription factors; artificial transcription; crispr; crispr dcas9 ... See more keywords
Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00101
Abstract: Inducible CRISPR-dCas9 transcription system has become a powerful tool for transcription regulation and sensing. Here, we develop a new concept of small-molecule-mediated split-aptamer assembly for inducible CRISPR-dCas9 transcription activation, allowing quantitative detection and imaging of… read more here.
Keywords: inducible crispr; dcas9 transcription; transcription; dcas9 ... See more keywords
Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00433
Abstract: Fluorescence microscopy imaging of specific chromosomal sites is essential for genome architecture research. To enable visualization of endogenous loci in mammalian cells, programmable DNA-binding proteins such as TAL effectors and CRISPR/dCas9 are commonly utilized. In… read more here.
Keywords: imaging method; replication; replication timing; crispr dcas9 ... See more keywords
P5387CRISPR/dCas9 Activated Expression of Cardiomyocyte Differentiation Factors in CDCs in Myocardial Infarctions
Sign Up to like & getrecommendations! Published in 2019 at "European Heart Journal"
DOI: 10.1093/eurheartj/ehz746.0347
Abstract: Existing therapies against myocardial infarction (MI) involve disease management by preventing additional damage to the heart muscle. However, new treatment strategies are in greater demand, which deems to focus on restoring cardiac function by replacing… read more here.
Keywords: differentiation factors; cdcs; expression; differentiation ... See more keywords
Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators
Sign Up to like & getrecommendations! Published in 2022 at "Horticulture Research"
DOI: 10.1093/hr/uhab037
Abstract: Abstract Overexpression and knockout (or knockdown) of gene of interest are two commonly used strategies for gene functional study. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene knockout has been applied in most plant… read more here.
Keywords: grape; activation; based transcriptional; gene ... See more keywords
P–567 Re-establishment of fertilization competency of the oocyte via CRISPR/dCas9 epigenome edition technology
Sign Up to like & getrecommendations! Published in 2021 at "Human Reproduction"
DOI: 10.1093/humrep/deab130.566
Abstract: Is it possible to increase the decreased levels of the sperm-oocyte binding protein, Juno, to restore the fertilization capacity of the oocyte, via the use of the CRISPR/dCas9 activation system? JUNO domain (in the oocyte)… read more here.
Keywords: fertilization; system; sperm binding; oocyte ... See more keywords
Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz709
Abstract: Abstract Establishing causal relationship between epigenetic marks and gene transcription requires molecular tools, which can precisely modify specific genomic regions. Here, we present a modular and extensible CRISPR/dCas9-based toolbox for epigenetic editing and direct gene… read more here.
Keywords: dcas9; system; dcas9 based; gene ... See more keywords
Enhancing the capability of Klebsiella pneumoniae to produce 1, 3‐propanediol by overexpression and regulation through CRISPR‐dCas9
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14033
Abstract: Klebsiella pneumoniae is a common strain of bacterial fermentation to produce 1, 3‐propanediol (1, 3‐PDO). In general, the production of 1, 3‐PDO by wild‐type K. pneumoniae is relatively low. Therefore, a new gene manipulation of… read more here.
Keywords: pdo; pneumoniae; production; pdo production ... See more keywords
Transcriptional activation of endogenous Oct4 via the CRISPR/dCas9 activator ameliorates Hutchinson-Gilford progeria syndrome in mice.
Sign Up to like & getrecommendations! Published in 2023 at "Aging cell"
DOI: 10.1111/acel.13825
Abstract: Partial cellular reprogramming via transient expression of Oct4, Sox2, Klf4, and c-Myc induces rejuvenation and reduces aged-cell phenotypes. In this study, we found that transcriptional activation of the endogenous Oct4 gene by using the CRISPR/dCas9… read more here.
Keywords: transcriptional activation; dcas9 activator; dcas9; crispr dcas9 ... See more keywords
CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing
Sign Up to like & getrecommendations! Published in 2019 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13232
Abstract: Summary Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9‐associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease‐dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in… read more here.
Keywords: crispr; dcas9 platforms; platforms plants; cas9 ... See more keywords