A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
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DOI: 10.1242/jcs.260000
Abstract: The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion/deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshift-promoting indels can… read more here.
Keywords: complete gene; gene knockout; crispr del; human diploid ... See more keywords