A new CRISPR-derived microRNA sensing mechanism based on Cas12a self-powered and rolling circle transcription (RCT)-unleashed real-time crRNA recruiting.
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DOI: 10.1021/acs.analchem.0c00680
Abstract: Current CRISPR-Cas-based nucleic acid sensing methods relying on the pre-assembled Cas-crRNA complexes are gen-erally limited to the detection of protospacer-adjacent motif (PAM)-containing sequences, and nonspecific back-grounds are inevitable. Herein, we propose a new CRISPR-derived microRNA… read more here.
Keywords: crispr; rct; cas12a; crispr derived ... See more keywords