Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis
Sign Up to like & getrecommendations! Published in 2024 at "Advanced Science"
DOI: 10.1002/advs.202402580
Abstract: Cas12j, a hypercompact and efficient Cas protein, has potential for use in CRISPR diagnostics, but has not yet been used because the trans‐cleavage activity of Cas12j is veiled. Here, the trans‐cleavage behavior of Cas12j1, 2,… read more here.
Keywords: detection; crispr diagnostics; trans cleavage; cleavage activity ... See more keywords
CRISPR Diagnostics: Advances toward the Point of Care.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00051
Abstract: CRISPR diagnostics have recently emerged as powerful diagnostic tools for the rapid detection of infections. The ultimate goal is to develop these diagnostics for the point of care, where patients quickly receive and easily interpret… read more here.
Keywords: point care; diagnostics advances; crispr diagnostics;
CRISPR diagnostics: Underappreciated uses in perinatology.
Sign Up to like & getrecommendations! Published in 2018 at "Seminars in perinatology"
DOI: 10.1053/j.semperi.2018.09.016
Abstract: CRISPR-based therapeutics have the potential to revolutionize the treatment of hereditary diseases, but current efforts to translate research to the bedside face significant technical, regulatory, and ethical hurdles. In this article, we discuss an underappreciated… read more here.
Keywords: crispr; uses perinatology; perinatology crispr; crispr diagnostics ... See more keywords
Sensitive and specific CRISPR diagnostics.
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.356.6336.392-h
Abstract: CRISPR Technology Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al. combined the allele-specific sensing ability of CRISPR-Cas13a with recombinase polymerase… read more here.
Keywords: crispr; science; specific crispr; sensitive specific ... See more keywords