CRISPR Editing in Biological and Biomedical Investigation
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DOI: 10.1002/jcb.26111
Abstract: The revolutionary technology for genome editing known as the clustered regularly interspaced short palindromic repeat (CRISPR)‐CRISPR‐associated protein 9 (Cas9) system has sparked advancements in biological and biomedical research. The scientific breakthrough of the development of… read more here.
Keywords: crispr; crispr cas9; crispr editing; cas9 system ... See more keywords
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.
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DOI: 10.1016/j.celrep.2020.107689
Abstract: CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence… read more here.
Keywords: target effects; crispr editing; detection deleterious; target ... See more keywords
CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with ARMS2/HTRA1-related AMD.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2215005120
Abstract: Genome-wide association studies (GWAS) have identified genetic risk loci for age-related macular degeneration (AMD) on the chromosome 10q26 (Chr10) locus and are tightly linked: the A69S (G>T) rs10490924 single-nucleotide variant (SNV) and the AATAA-rich insertion-deletion… read more here.
Keywords: stress; editing demonstrates; crispr editing; oxidative stress ... See more keywords
CRISPR editing of CCR5 and HIV-1 facilitates viral elimination in antiretroviral drug-suppressed virus-infected humanized mice
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2217887120
Abstract: Significance Long-acting antiretroviral drugs suppress viral replication in humanized mice. When administered CRISPR-mediated CCR5 and HIV-1LTR-Gag CRISPR editing the treatments resulted in the elimination of HIV-1 proviral DNA in lymphoid, bone marrow, and central nervous… read more here.
Keywords: hiv; crispr; humanized mice; crispr editing ... See more keywords