Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.01.002
Abstract: Mutagenesis studies have rapidly evolved in the era of CRISPR genome editing. Precise manipulation of genes in human induced pluripotent stem cells (iPSCs) allows biomedical researchers to study the physiological functions of individual genes during… read more here.
Keywords: genome editing; human ipscs; targeted mutagenesis; crispr genome ... See more keywords
CRISPR genome editing in stem cells turns to gold
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DOI: 10.1038/s41563-019-0491-4
Abstract: Hematopoietic stem and progenitor cells have been engineered using gold nanoformulations conjugated with CRISPR capable of targeting two distinct genomic loci of therapeutic interest, with potential engraftment in humanized mouse models. read more here.
Keywords: genome editing; crispr genome; cells turns; stem ... See more keywords
Complementary CRISPR genome-wide genetic screens in PARP10-knockout and overexpressing cells identify synthetic interactions for PARP10-mediated cellular survival
Sign Up to like & getrecommendations! Published in 2022 at "Oncotarget"
DOI: 10.18632/oncotarget.28277
Abstract: PARP10 is a mono-ADP-ribosyltransferase with multiple cellular functions, including proliferation, apoptosis, metabolism and DNA repair. PARP10 is overexpressed in a significant proportion of tumors, particularly breast and ovarian cancers. Identifying genetic susceptibilities based on PARP10… read more here.
Keywords: genome wide; mediated cellular; parp10; parp10 mediated ... See more keywords