CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
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DOI: 10.1038/s41467-020-17952-5
Abstract: Precise genome editing using CRISPR-Cas9 is a promising therapeutic avenue for genetic diseases, although off-target editing remains a significant safety concern. Guide RNAs shorter than 16 nucleotides in length effectively recruit Cas9 to complementary sites… read more here.
Keywords: crispr guard; guide rnas; target; activity ... See more keywords