CRISPR Interference and Activation to Modulate Transcription in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1414-3_6
Abstract: Recent developments in RNA-guided nuclease technologies have advanced the engineering of a wide range of organisms, including the nonconventional yeast Yarrowia lipolytica. Y. lipolytica has been the focus of a range of synthetic biology and… read more here.
Keywords: transcription; crispr interference; biology; activation ... See more keywords
Inhibition of KU70 and KU80 by CRISPR interference, not NgAgo interference, increases the efficiency of homologous recombination in pig fetal fibroblasts
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Integrative Agriculture"
DOI: 10.1016/s2095-3119(18)62150-1
Abstract: Abstract Non-homologous end-joining (NHEJ) is a predominant pathway for the repair of DNA double-strand breaks (DSB). It inhibits the efficiency of homologous recombination (HR) by competing for DSB targets. To improve the efficiency of HR,… read more here.
Keywords: efficiency homologous; ku70 ku80; homologous recombination; interference ... See more keywords
A multiplex CRISPR interference tool for virulence gene interrogation in Legionella pneumophila
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-01672-7
Abstract: Catalytically inactive dCas9 imposes transcriptional gene repression by sterically precluding RNA polymerase activity at a given gene to which it was directed by CRISPR (cr)RNAs. This gene silencing technology, known as CRISPR interference (CRISPRi), has… read more here.
Keywords: virulence; gene; crispr interference; legionella pneumophila ... See more keywords
Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance.
Sign Up to like & getrecommendations! Published in 2019 at "Genome research"
DOI: 10.1101/gr.246900.118
Abstract: Obesity is an increasing pathophysiological problem in developed societies. Despite all major progress in understanding molecular mechanisms of obesity, currently available anti-obesity drugs have shown limited efficacy with severe side effects. CRISPR interference (CRISPRi) mechanism… read more here.
Keywords: crispr interference; system fabp4; delivery; obesity ... See more keywords
CRISPR interference (CRISPRi) as transcriptional repression tool for Hungateiclostridium thermocellum DSM 1313
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DOI: 10.1111/1751-7915.13516
Abstract: Hungateiclostridium thermocellum DSM 1313 has biotechnological potential as a whole‐cell biocatalyst for ethanol production using lignocellulosic renewable sources. The full exploitation of H. thermocellum has been hampered due to the lack of simple and high‐throughput… read more here.
Keywords: hungateiclostridium thermocellum; dsm 1313; crispr interference; thermocellum dsm ... See more keywords
Efficient Suppression of Natural Plasmid-Borne Gene Expression in Carbapenem-Resistant Klebsiella pneumoniae Using a Compact CRISPR Interference System
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DOI: 10.1128/aac.00890-22
Abstract: There is an urgent need for efficient tools for genetic manipulation to assess plasmid function in clinical drug-resistant bacterial strains. To address this need, we developed an all-in-one CRISPR interference (CRISPRi) system that easily inhibited… read more here.
Keywords: system; pneumoniae; plasmid; crispr interference ... See more keywords
CRISPR Interference Reveals That All-Trans-Retinoic Acid Promotes Macrophage Control of Mycobacterium tuberculosis by Limiting Bacterial Access to Cholesterol and Propionyl Coenzyme A
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DOI: 10.1128/mbio.03683-21
Abstract: Tuberculosis, caused by the bacterium Mycobacterium tuberculosis, is a leading cause of death due to infectious disease. Improving the immune response to tuberculosis holds promise for fighting the disease but is limited by our lack… read more here.
Keywords: crispr interference; tuberculosis; cholesterol; mycobacterium tuberculosis ... See more keywords
A Genome-Wide CRISPR Interference Screen Reveals an StkP-Mediated Connection between Cell Wall Integrity and Competence in Streptococcus salivarius
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DOI: 10.1128/msystems.00735-22
Abstract: Survival of human commensal streptococci in the digestive tract requires efficient strategies which must be tightly and collectively controlled for responding to competitive pressure and drastic environmental changes. In this context, the autocrine signaling system… read more here.
Keywords: competence; cell; crispr interference; cell wall ... See more keywords
CRISPR Interference-Based Inhibition of MAB_0055c Expression Alters Drug Sensitivity in Mycobacterium abscessus
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DOI: 10.1128/spectrum.00631-23
Abstract: In this study, we utilized CRISPR interference (CRISPRi) to specifically target the MAB_0055c gene in M. abscessus, a bacterium that causes difficult-to-treat infections. The study found that silencing the gene lead to increased rifabutin and… read more here.
Keywords: gene; mab 0055c; crispr interference; abscessus ... See more keywords
Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cell science"
DOI: 10.1242/jcs.261186
Abstract: Characterizing functions of essential genes is challenging, as perturbing them is generally lethal. Conditional gene perturbation, including use of temperature-sensitive mutants, has been widely utilized to reveal functions of essential genes in the fission yeast… read more here.
Keywords: conserved essential; crispr interference; fission yeast; essential genes ... See more keywords
A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1009103
Abstract: G protein-coupled receptors (GPCRs) allow cells to respond to chemical and sensory stimuli through generation of second messengers, such as cyclic AMP (cAMP), which in turn mediate a myriad of processes, including cell survival, proliferation,… read more here.
Keywords: crispr interference; gpcr camp; camp; camp signaling ... See more keywords