Functional Phosphoproteomics in Cancer Chemoresistance Using CRISPR‐Mediated Base Editors
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202200717
Abstract: Selective inhibition of targeted protein kinases is an effective therapeutic approach for treatment of human malignancies, which interferes phosphorylation of cellular substrates. However, a drug‐imposed selection creates pressures for tumor cells to acquire chemoresistance‐conferring mutations… read more here.
Keywords: crispr mediated; functional phosphoproteomics; phosphorylation; chemoresistance ... See more keywords
CRISPR‐Mediated Enzyme Fragment Complementation Assay for Quantification of the Stability of Splice Isoforms
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200012
Abstract: Small‐molecule splicing modulators exemplified by an FDA‐approved drug, risdiplam, are a new pharmacological modality for regulating the expression and stability of splice isoforms. We report a CRISPR‐mediated enzyme fragment complementation (EFC) assay to quantify the… read more here.
Keywords: complementation; efc assay; stability splice; crispr mediated ... See more keywords
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.06.064
Abstract: The CRISPR/Cas9 system has recently been shown to facilitate high levels of precise genome editing using adeno-associated viral (AAV) vectors to serve as donor template DNA during homologous recombination (HR). However, the maximum AAV packaging capacity… read more here.
Keywords: genome editing; integration; crispr mediated; donor ... See more keywords
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.09.029
Abstract: The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of a range of DNA and chromatin-modifying enzymes. While homology-directed… read more here.
Keywords: base editing; mediated base; methods applications; crispr mediated ... See more keywords
Conditional CRISPR‐mediated deletion of Lyn kinase enhances differentiation and function of iPSC‐derived megakaryocytes
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Thrombosis and Haemostasis"
DOI: 10.1111/jth.15546
Abstract: Thrombocytopenia leading to life‐threatening excessive bleeding can be treated by platelet transfusion. Currently, such treatments are totally dependent on donor‐derived platelets. To support future applications in the use of in vitro‐derived platelets, we sought to… read more here.
Keywords: conditional crispr; crispr mediated; deletion lyn; lyn kinase ... See more keywords
CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii
Sign Up to like & getrecommendations! Published in 2021 at "mSphere"
DOI: 10.1128/msphere.00474-21
Abstract: Toxoplasma gondii is a ubiquitous intracellular parasite of humans and animals that causes life-threatening disease in immunocompromised patients, fetal abnormalities when contracted during gestation, and recurrent eye lesions in some patients. Despite its health implications,… read more here.
Keywords: toxoplasma gondii; crispr mediated; toxoplasma; gene ... See more keywords
Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells.
Sign Up to like & getrecommendations! Published in 2019 at "Wellcome open research"
DOI: 10.12688/wellcomeopenres.15119.3
Abstract: Human pluripotent stem cells are increasingly used for CRISPR-mediated gene targeting in efforts to generate models of human diseases. This is a challenging task because of the high sensitivity of these cells to suboptimal conditions,… read more here.
Keywords: crispr; crispr mediated; screening crispr; pluripotent stem ... See more keywords
beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing
Sign Up to like & getrecommendations! Published in 2019 at "Genetics"
DOI: 10.1534/genetics.119.302089
Abstract: The biological relevance of the present method pertains to CRISPR-mediated base editing technology that has opened new avenues for scar-free genome-wide mutagenesis and thus to a wide range of applications in genome editing... CRISPR-mediated base… read more here.
Keywords: base editing; mediated base; guide rnas; crispr mediated ... See more keywords
A Review on CRISPR-Mediated Epigenome Editing: A Future Directive for Therapeutic Management of Cancer.
Sign Up to like & getrecommendations! Published in 2022 at "Current drug targets"
DOI: 10.2174/1389450123666220117105531
Abstract: Recent studies have shed light on the role of epigenetic marks in certain diseases like cancer, type II diabetes mellitus (T2DM), obesity, and cardiovascular dysfunction, to name a few. Epigenetic marks like DNA methylation and… read more here.
Keywords: crispr; mediated epigenome; crispr mediated; review crispr ... See more keywords
Utilization of CRISPR-Mediated Tools for Studying Functional Genomics in Hematological Malignancies: An Overview on the Current Perspectives, Challenges, and Clinical Implications
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2021.767298
Abstract: Hematological malignancies (HM) are a group of neoplastic diseases that are usually heterogenous in nature due to the complex underlying genetic aberrations in which collaborating mutations enable cells to evade checkpoints that normally safeguard it… read more here.
Keywords: crispr; mediated tools; hematological malignancies; crispr mediated ... See more keywords
Non-Transgenic CRISPR-Mediated Knockout of Entire Ergot Alkaloid Gene Clusters in Slow-Growing Asexual Polyploid Fungi
Sign Up to like & getrecommendations! Published in 2021 at "Toxins"
DOI: 10.3390/toxins13020153
Abstract: The Epichloë species of fungi include seed-borne symbionts (endophytes) of cool-season grasses that enhance plant fitness, although some also produce alkaloids that are toxic to livestock. Selected or mutated toxin-free endophytes can be introduced into… read more here.
Keywords: crispr; transgenic crispr; crispr mediated; non transgenic ... See more keywords