An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.014
Abstract: The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can… read more here.
Keywords: transcription translation; free transcription; cell free; translation system ... See more keywords
Anti-CRISPRdb v2.2: an online repository of anti-CRISPR proteins including information on inhibitory mechanisms, activities and neighbors of curated anti-CRISPR proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Database : the journal of biological databases and curation"
DOI: 10.1093/database/baac010
Abstract: We previously released the Anti-CRISPRdb database hosting anti-CRISPR proteins (Acrs) and associated information. Since then, the number of known Acr families, types, structures and inhibitory activities has accumulated over time, and Acr neighbors can be… read more here.
Keywords: crispr proteins; crispr; information; anti crisprdb ... See more keywords
Phages, anti-CRISPR proteins, and drug-resistant bacteria: what do we know about this triad?
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens and disease"
DOI: 10.1093/femspd/ftac039
Abstract: Phages are viruses that infect bacteria, relying on their genetic machinery to replicate. To survive the constant attack of phages, bacteria have developed diverse defense strategies to act against them. Nevertheless, phages rapidly co-evolve to… read more here.
Keywords: proteins drug; crispr proteins; anti crispr; phages anti ... See more keywords
Expanding the application of anti-CRISPR proteins in plants for tunable genome editing
Sign Up to like & getrecommendations! Published in 2023 at "Plant Physiology"
DOI: 10.1093/plphys/kiad076
Abstract: Anti-CRISPR proteins are very efficient for inhibiting CRISPR/Cas9-based genome editing tools in both herbaceous and woody plant species. read more here.
Keywords: genome editing; crispr proteins; anti crispr; proteins plants ... See more keywords
Gene drive inhibition by the anti-CRISPR proteins AcrIIA2 and AcrIIA4 in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2018 at "Microbiology"
DOI: 10.1099/mic.0.000635
Abstract: Given the widespread use and application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas gene editing system across many fields, a major focus has been the development, engineering and discovery of molecular means to… read more here.
Keywords: acriia2 acriia4; gene drive; anti crispr; gene ... See more keywords
Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins.
Sign Up to like & getrecommendations! Published in 2019 at "RNA"
DOI: 10.1261/rna.071704.119
Abstract: CRISPR-Cas systems are bacterial adaptive immune pathways that have revolutionized biotechnology and biomedical applications. Despite the potential for human therapeutic development, there are many hurdles that must be overcome before its use in clinical settings.… read more here.
Keywords: genome editing; vivo; tissue; anti crispr ... See more keywords
In silico approaches for prediction of anti-CRISPR proteins.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of molecular biology"
DOI: 10.2139/ssrn.4259856
Abstract: Numerous viruses infecting bacteria and archaea encode CRISPR-Cas system inhibitors, known as anti-CRISPR proteins (Acr). The Acrs typically are highly specific for particular CRISPR variants, resulting in remarkable sequence and structural diversity and complicating accurate… read more here.
Keywords: crispr proteins; crispr; anti crispr; prediction ... See more keywords