A Genome-wide CRISPR Screen Identifies ZCCHC14 as a Host Factor Required for Hepatitis B Surface Antigen Production.
Sign Up to like & getrecommendations! Published in 2019 at "Cell reports"
DOI: 10.1016/j.celrep.2019.10.113
Abstract: A hallmark of chronic hepatitis B (CHB) virus infection is the presence of high circulating levels of non-infectious small lipid HBV surface antigen (HBsAg) vesicles. Although rare, sustained HBsAg loss is the idealized endpoint of… read more here.
Keywords: genome wide; surface antigen; hepatitis; wide crispr ... See more keywords
A FACS-Based Genome-wide CRISPR Screen Reveals a Requirement for COPI in Chlamydia trachomatis Invasion
Sign Up to like & getrecommendations! Published in 2019 at "iScience"
DOI: 10.1016/j.isci.2018.12.011
Abstract: The invasion of Chlamydia trachomatis, an obligate intracellular bacterium, into epithelial cells is driven by a complex interplay of host and bacterial factors. To comprehensively define the host genes required for pathogen invasion, we undertook… read more here.
Keywords: invasion; chlamydia trachomatis; facs based; trachomatis ... See more keywords
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
Sign Up to like & getrecommendations! Published in 2017 at "Nature neuroscience"
DOI: 10.1038/nn.4620
Abstract: A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus–mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly… read more here.
Keywords: mediated direct; crispr; direct vivo; aav mediated ... See more keywords
Identification of splice regulators of fibronectin-EIIIA and EIIIB by direct measurement of exon usage in a flow-cytometry based CRISPR screen
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-99079-1
Abstract: The extracellular matrix protein fibronectin (FN) is alternatively spliced in a variety of inflammatory conditions, resulting in increased inclusion of alternative exons EIIIA and EIIIB. Inclusion of these exons affects fibril formation, fibrosis, and inflammation.… read more here.
Keywords: inclusion; eiiia eiiib; flow cytometry; cytometry based ... See more keywords
Genome-wide CRISPR screen identifies protein pathways modulating tau protein levels in neurons
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-02272-1
Abstract: Aggregates of hyperphosphorylated tau protein are a pathological hallmark of more than 20 distinct neurodegenerative diseases, including Alzheimer’s disease, progressive supranuclear palsy, and frontotemporal dementia. While the exact mechanism of tau aggregation is unknown, the… read more here.
Keywords: crispr screen; genome wide; wide crispr; tau protein ... See more keywords
Abstract 2819: Genome-wide CRISPR screen identifies NANP as a radio-sensitizing target of GBM by regulating NF-κB pathway
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-2819
Abstract: Glioblastoma multiforme (GBM) are the most malignant primary central nervous system tumors. The standard of care for newly diagnosed GBM patients includes surgery followed by combined radiation therapy (RT) and adjuvant temozolomide (TMZ) therapy. However,… read more here.
Keywords: crispr screen; genome wide; radio sensitizing; sensitizing target ... See more keywords
Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells
Sign Up to like & getrecommendations! Published in 2019 at "BMC Cancer"
DOI: 10.1186/s12885-019-5455-1
Abstract: BackgroundDespite its relatively low incidence, pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer deaths because of the aggressive growth/metastasis of the tumor, the lack of early symptoms, and the poor treatment options. Basic… read more here.
Keywords: genome wide; wide crispr; screen; cancer ... See more keywords
A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery
Sign Up to like & getrecommendations! Published in 2018 at "Retrovirology"
DOI: 10.1186/s12977-018-0409-2
Abstract: The InterFeron Induced TransMembrane (IFITM) proteins are interferon stimulated genes that restrict many viruses, including HIV-1. SAMHD1 is another restriction factor blocking replication of HIV-1 and other viruses. Some lentiviruses evolved Vpx/Vpr proteins to degrade… read more here.
Keywords: crispr; delivery; hiv; screen factors ... See more keywords
Surfaceome CRISPR screen identifies OLFML3 as a rhinovirus-inducible IFN antagonist
Sign Up to like & getrecommendations! Published in 2021 at "Genome Biology"
DOI: 10.1186/s13059-021-02513-w
Abstract: Background Rhinoviruses (RVs) cause more than half of common colds and, in some cases, more severe diseases. Functional genomics analyses of RVs using siRNA or genome-wide CRISPR screen uncovered a limited set of host factors,… read more here.
Keywords: genome wide; screen; host; crispr screen ... See more keywords
CRISPR Screens in Synthetic Lethality and Combinatorial Therapies for Cancer
Sign Up to like & getrecommendations! Published in 2021 at "Cancers"
DOI: 10.3390/cancers13071591
Abstract: Simple Summary The synthetic lethality (SL) clinical success of PARP inhibitors in homologous recombinant deficient tumors has established a new concept for cancer treatment. For decades, efforts have centered on identifying genetic interactions for determining… read more here.
Keywords: methodology; crispr; synthetic lethality; cancer ... See more keywords