Single-cell CRISPR screening in drug resistance
Sign Up to like & getrecommendations! Published in 2017 at "Cell Biology and Toxicology"
DOI: 10.1007/s10565-017-9396-7
Abstract: Development of resistance and insensitivity to target therapy is a growing challenge during clinical treatment for patients with cancer that may require solutions via genome medicine (Gu & Wang 2017). Gene changes such as mutations… read more here.
Keywords: single cell; drug resistance; resistance; crispr screening ... See more keywords
In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer
Sign Up to like & getrecommendations! Published in 2021 at "Cell"
DOI: 10.1016/j.cell.2021.02.019
Abstract: Improving effector activity of antigen-specific T cells is a major goal in cancer immunotherapy. Despite the identification of several effector T cell (TEFF)-driving transcription factors (TFs), the transcriptional coordination of TEFF biology remains poorly understood. We developed… read more here.
Keywords: cd8; cell crispr; biology; cancer ... See more keywords
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions
Sign Up to like & getrecommendations! Published in 2021 at "Cell"
DOI: 10.1016/j.cell.2021.02.021
Abstract: How early events in effector T cell (TEFF) subsets tune memory T cell (TMEM) responses remains incompletely understood. Here, we systematically investigated metabolic factors in fate determination of TEFF and TMEM cells using in vivo pooled CRISPR screening,… read more here.
Keywords: tmem; crispr screening; cell fate; nutrient signaling ... See more keywords
Combination therapy for HCC: from CRISPR screening to the design of clinical therapies
Sign Up to like & getrecommendations! Published in 2021 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-021-00775-1
Abstract: CRISPR screening has been broadly used to discover new therapy targets, but these finding’ successful clinical transition is still limited. In a recent study published in Nature, Jin et al. adopted CRISPR screening to dissect… read more here.
Keywords: combination; lenvatinib; hcc; therapy ... See more keywords
Model-based understanding of single-cell CRISPR screening
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-10216-x
Abstract: The recently developed single-cell CRISPR screening techniques, independently termed Perturb-Seq, CRISP-seq, or CROP-seq, combine pooled CRISPR screening with single-cell RNA-seq to investigate functional CRISPR screening in a single-cell granularity. Here, we present MUSIC, an integrated… read more here.
Keywords: single cell; model based; cell crispr; crispr screening ... See more keywords
Genome-wide CRISPR screening uncovers potential targets and mechanisms of vincristine resistance in DLBCL.
Sign Up to like & getrecommendations! Published in 2023 at "British journal of haematology"
DOI: 10.1111/bjh.18900
Abstract: In this issue, Rovsing et al. employ unbiased genome-wide CRISPR screening and functional cellular assays to investigate the cellular response to vincristine, an important component of the front-line DLBCL treatment R-CHOP. Their findings reveal intriguing… read more here.
Keywords: genome wide; dlbcl; wide crispr; crispr screening ... See more keywords
SPOT-012 Large-scale CRISPR screening to identify actionable cancer drug targets
Sign Up to like & getrecommendations! Published in 2018 at "ESMO Open"
DOI: 10.1136/esmoopen-2018-eacr25.45
Abstract: Introduction In order to identify new putative oncology drug targets, we have developed a whole-genome CRISPR drop-out screening pipeline. Material and methods The CRISPR drop-out library used in this pipeline is composed of 1 01 094 single… read more here.
Keywords: drug targets; drug; cancer; crispr screening ... See more keywords
In vivo genome-wide CRISPR screening in murine acute myeloid leukemia uncovers microenvironmental dependencies
Sign Up to like & getrecommendations! Published in 2022 at "Blood Advances"
DOI: 10.1182/bloodadvances.2022007250
Abstract: Key Points In vivo CRISPR screens in AML define key interactors of the microenvironment, including integrins, immune modulators, and glycosylation. Eight in vivo–specific hits are recurrently associated with adverse prognosis: BTBD6, FERMT3, ILK, SLC19A1, TAP2,… read more here.
Keywords: crispr; screening murine; genome wide; wide crispr ... See more keywords
Integrative analysis of CRISPR screening data uncovers new opportunities for optimizing cancer immunotherapy
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Cancer"
DOI: 10.1186/s12943-021-01462-z
Abstract: Background In recent years, the application of functional genetic immuno-oncology screens has showcased the striking ability to identify potential regulators engaged in tumor-immune interactions. Although these screens have yielded substantial data, few studies have attempted… read more here.
Keywords: tumor; analysis; crispr screening; oncology ... See more keywords
Genome-scale CRISPR screening for modifiers of cellular LDL uptake.
Sign Up to like & getrecommendations! Published in 2021 at "PLoS genetics"
DOI: 10.1371/journal.pgen.1009285
Abstract: Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale… read more here.
Keywords: genome scale; ldl uptake; scale crispr; cellular ldl ... See more keywords
High-content CRISPR screening in tumor immunology
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.1041451
Abstract: CRISPR screening is a powerful tool that links specific genetic alterations to corresponding phenotypes, thus allowing for high-throughput identification of novel gene functions. Pooled CRISPR screens have enabled discovery of innate and adaptive immune response… read more here.
Keywords: tumor immunology; crispr; high content; crispr screening ... See more keywords