Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_457
Abstract: Pooled CRISPR screens are emerging as a powerful tool to dissect regulatory networks, by assessing how a protein responds to genetic perturbations in a highly multiplexed manner. A large number of genes are perturbed in… read more here.
Keywords: molecular phenotypes; crispr screens; facs based; dissecting molecular ... See more keywords
Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2019.07.044
Abstract: CD8 T cells play essential roles in anti-tumor immune responses. Here, we performed genome-scale CRISPR screens in CD8 T cells directly under cancer immunotherapy settings and identified regulators of tumor infiltration and degranulation. The in vivo screen robustly re-identified… read more here.
Keywords: genome scale; immunotherapy; immunotherapy target; cd8 cells ... See more keywords
Corrigendum: Genome-wide CRISPR screens reveal a Wnt–FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors
Sign Up to like & getrecommendations! Published in 2017 at "Nature Medicine"
DOI: 10.1038/nm1117-1384d
Abstract: This corrects the article DOI: 10.1038/nm.4219 read more here.
Keywords: reveal wnt; genome wide; wide crispr; corrigendum genome ... See more keywords
Analysis of combinatorial CRISPR screens with the Orthrus scoring pipeline.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00596-0
Abstract: The continued improvement of combinatorial CRISPR screening platforms necessitates the development of new computational pipelines for scoring combinatorial screening data. Unlike for single-guide RNA (sgRNA) pooled screening platforms, combinatorial scoring for multiplexed systems is confounded… read more here.
Keywords: crispr; screens orthrus; combinatorial crispr; screening data ... See more keywords
Interrogating bromodomain inhibitor resistance in KMT2A-rearranged leukemia through combinatorial CRISPR screens
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2220134120
Abstract: Significance Using combinatorial loss-of-function CRISPR screens, our study revealed a Bromo- and extra-terminal domain inhibitors (BETi) resistance network in KMT2A-r leukemia. Based on these findings, we have also revealed a corresponding synergy treatment regimen, significantly… read more here.
Keywords: inhibitor resistance; crispr screens; interrogating bromodomain; resistance ... See more keywords
SCREE: a comprehensive pipeline for single-cell multi-modal CRISPR screen data processing and analysis.
Sign Up to like & getrecommendations! Published in 2023 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbad123
Abstract: Single-cell CRISPR screens have been widely used to investigate gene regulatory circuits in diverse biological systems. The recent development of single-cell CRISPR screens has enabled multimodal profiling of perturbed cells with both gene expression, chromatin… read more here.
Keywords: cell; cell crispr; crispr screens; single cell ... See more keywords
In vivo CRISPR screens identify RhoV as a pro‐metastasis factor of triple‐negative breast cancer
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Science"
DOI: 10.1111/cas.15783
Abstract: Metastasis is the main death reason for triple‐negative breast cancer (TNBC). Thus, identifying the driver genes associated with metastasis of TNBC is urgently needed. CRISPR screens have dramatically enhanced genome editing and made it possible… read more here.
Keywords: metastasis; crispr screens; rhov; tnbc ... See more keywords
In vivo multidimensional CRISPR screens identify Lgals2 as an immunotherapy target in triple-negative breast cancer
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abl8247
Abstract: Immune checkpoint inhibitors exhibit limited response rates in patients with triple-negative breast cancer (TNBC), suggesting that additional immune escape mechanisms may exist. Here, we performed two-step customized in vivo CRISPR screens targeting disease-related immune genes… read more here.
Keywords: immunotherapy target; immunotherapy; crispr screens; triple negative ... See more keywords
High-Throughput CRISPR Screens To Dissect Macrophage-Shigella Interactions
Sign Up to like & getrecommendations! Published in 2021 at "mBio"
DOI: 10.1128/mbio.02158-21
Abstract: Treatment for shigellosis is becoming increasingly difficult as resistance to antibiotics becomes more prevalent. One way to prevent this significant public health problem from developing into a full-blown crisis is to approach shigellosis intervention from… read more here.
Keywords: crispr screens; shigella; high throughput; shigellosis ... See more keywords
Abstract 390: Genome-wide CRISPR screens in brain tumor initiating cells (BTICs) identify potent sensitizers and resistors of conventional chemoradiotherapy
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-390
Abstract: Glioblastoma (GBM) is a highly aggressive and most common form of malignant primary brain tumors in adults (WHO grade IV). Despite surgical and therapeutic interventions, including chemotherapy with the alkylating agent Temozolomide (TMZ) and cranial… read more here.
Keywords: genome wide; wide crispr; screens brain; brain tumor ... See more keywords
Abstract LB341: Single-cell CRISPR screens in primary human T cells identify regulators of Th2 cell skewing
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-lb341
Abstract: CRISPR screens have become the primary discovery engine in modern biology. However, many screening workflows are still performed in cancer cell lines and coupled to simplistic read-outs such as cellular fitness. At Myllia Biotechnology, we… read more here.
Keywords: cell; primary human; human cells; crispr screens ... See more keywords