The Manipulation of RNA‐Guided Nucleic Acid Cleavage with Ninhydrin Chemistry
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.201903770
Abstract: Abstract CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome‐editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need… read more here.
Keywords: crispr systems; ninhydrin chemistry; rna guided; chemistry ... See more keywords
Beyond Native Cas9: Manipulating Genomic Information and Function.
Sign Up to like & getrecommendations! Published in 2017 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2017.06.004
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated manipulation of genomic information is becoming more versatile by combining nuclease-deficient CRISPR systems with a wide variety of effectors including base-editing deaminases, transcriptional regulators, and epigenetic modifiers. The… read more here.
Keywords: information; crispr systems; genomic information; beyond native ... See more keywords
Type III-A CRISPR immunity promotes mutagenesis of staphylococci.
Sign Up to like & getrecommendations! Published in 2021 at "Nature"
DOI: 10.1038/s41586-021-03440-3
Abstract: Horizontal gene transfer and mutation are the two major drivers of microbial evolution that enable bacteria to adapt to fluctuating environmental stressors1. Clustered, regularly interspaced, short palindromic repeats (CRISPR) systems use RNA-guided nucleases to direct… read more here.
Keywords: iii crispr; crispr systems; type iii;
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
Sign Up to like & getrecommendations! Published in 2019 at "Nature biotechnology"
DOI: 10.1038/s41587-019-0095-1
Abstract: CRISPR (clustered regularly interspaced short palindromic repeat) systems have been broadly adopted for basic science, biotechnology, and gene and cell therapy. In some cases, these bacterial nucleases have demonstrated off-target activity. This creates a potential… read more here.
Keywords: rna secondary; crispr systems; crispr; increasing specificity ... See more keywords
The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2150812
Abstract: ABSTRACT CRISPR systems elicit interference when a foreign nucleic acid is detected by its ability to base-pair to crRNA. Understanding what degree of complementarity between a foreign nucleic acid and crRNA is required for interference… read more here.
Keywords: type iii; crrna; interference; crispr systems ... See more keywords
Genomic Insights into CRISPR-Harboring Plasmids in the Klebsiella Genus: Distribution, Backbone Structures, Antibiotic Resistance, and Virulence Determinant Profiles
Sign Up to like & getrecommendations! Published in 2023 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.01189-22
Abstract: CRISPR systems are often encoded by many prokaryotes as adaptive defense against mobile genetic elements (MGEs), but several MGEs also recruit CRISPR components to perform additional biological functions. Type IV-A systems are identified in Klebsiella… read more here.
Keywords: crispr harboring; klebsiella genus; klebsiella; crispr systems ... See more keywords
Evolutionary plasticity and functional versatility of CRISPR systems
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3001481
Abstract: The principal biological function of bacterial and archaeal CRISPR systems is RNA-guided adaptive immunity against viruses and other mobile genetic elements (MGEs). These systems show remarkable evolutionary plasticity and functional versatility at multiple levels, including… read more here.
Keywords: crispr; functional versatility; crispr systems; plasticity functional ... See more keywords