Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-72464-y
Abstract: Plants are sessile organisms, which are vulnerable to environmental stresses. As such, plants have developed multiple molecular, physiological, and cellular mechanisms to cope with natural stressors. However, these environmental adversities, including drought, are sources of… read more here.
Keywords: severe water; plant; water; water deficit ... See more keywords
High throughput CRISPRi and CRISPRa technologies in 3D genome regulation for neuropsychiatric diseases.
Sign Up to like & getrecommendations! Published in 2022 at "Human molecular genetics"
DOI: 10.1093/hmg/ddac193
Abstract: Advances in genomics have led to the identification of many risk loci with hundreds of genes and thousands of DNA variants associated with neuropsychiatric disorders. A significant barrier to understanding the genetic underpinnings of complex… read more here.
Keywords: neuropsychiatric diseases; crispra; high throughput; crispri crispra ... See more keywords
CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac804
Abstract: Abstract Governance of the endogenous gene regulatory network enables the navigation of cells towards beneficial traits for recombinant protein production. CRISPRactivation and interference provides the basis for gene expression modulation but is primarily applied in… read more here.
Keywords: escherichia coli; crispra; pam sequence; regulation ... See more keywords
CRISPRa corrects haploinsufficient obesity
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DOI: 10.1126/science.363.6424.241-l
Abstract: Gene Therapy Loss-of-function mutation in one gene copy, termed haploinsufficiency, can lead to insufficient protein levels and result in human disease. Matharu et al. tested whether a CRISPR-based activation system (CRISPRa) could rescue a haploinsufficient… read more here.
Keywords: science; haploinsufficient obesity; corrects haploinsufficient; obesity ... See more keywords
Promoting CRISPRa for Targeted Treatment of Epilepsy
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DOI: 10.1177/1535759720935825
Abstract: In Vivo CRISPRa Decreases Seizures and Rescues Cognitive Deficits in a Rodent Model of Epilepsy Colasante G, Qiu Y, Massimino L, et al. Brain. 2020;143(3):891-905. doi:10.1093/brain/awaa045 Epilepsy is a major health burden, calling for new… read more here.
Keywords: epilepsy; promoting crispra; crispra targeted; crispra ... See more keywords
Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activation
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DOI: 10.1371/journal.pone.0270008
Abstract: CRISPR/Cas9-mediated transcriptional activation (CRISPRa) is a powerful tool for investigating complex biological phenomena. Although CRISPRa approaches based on the VP64 transcriptional activator have been widely studied in both cultured cells and in animal models and… read more here.
Keywords: dcas9 vp64; activation; crispra; dcas9 ... See more keywords
Clearing of Foreign Episomal DNA from Human Cells by CRISPRa-Mediated Activation of Cytidine Deaminases
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21186865
Abstract: Restriction of foreign DNA is a fundamental defense mechanism required for maintaining genomic stability and proper function of mammalian cells. APOBEC cytidine deaminases are crucial effector molecules involved in clearing pathogenic DNA of viruses and… read more here.
Keywords: crispra mediated; dna; crispra; cytidine deaminases ... See more keywords