CRISPRi repression of nonhomologous end‐joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26404
Abstract: In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most… read more here.
Keywords: homologous recombination; repression; nonhomologous end; crispri ... See more keywords
Controlling microbial PHB synthesis via CRISPRi
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8374-6
Abstract: Microbial polyhydroxyalkanoates (PHA) are a family of biopolyesters with properties similar to petroleum plastics such as polyethylene (PE) or polypropylene (PP). Polyhydroxybutyrate (PHB) is the most common PHA known so far. Clustered regularly interspaced short… read more here.
Keywords: synthesis; crispri; phb synthesis; phac activity ... See more keywords
Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9.
Sign Up to like & getrecommendations! Published in 2022 at "Stem cell research"
DOI: 10.1016/j.scr.2022.102754
Abstract: Temporal regulation of CRISPRi activity is critical for genetic screens. Here, we present an inducible CRISPRi platform enabling selection of iPSC-derived cardiomyocytes and reversible gene knockdown. We targeted a doxycycline-inducible dCas9-KRAB-mCherry cassette into the AAVS1… read more here.
Keywords: myl7; dcas9; ipsc; stem cell ... See more keywords
Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.8b00429
Abstract: CRISPR interference (CRISPRi) via target guide RNA (gRNA) arrays and a deactivated Cas9 (dCas9) protein has been shown to simultaneously repress expression of multiple genomic DNA loci. By knocking down endogenous genes in competing pathways,… read more here.
Keywords: metabolic flux; production; repression; crispri mediated ... See more keywords
Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux
Sign Up to like & getrecommendations! Published in 2021 at "Communications Biology"
DOI: 10.1038/s42003-021-02541-z
Abstract: The haloarchaeon Haloferax mediterranei is a potential strain for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production, yet the production yield and cost are the major obstacles hindering the use of this archaeal strain. Leveraging the endogenous type I-B CRISPR-Cas… read more here.
Keywords: production; metabolic pathways; optimising phbv; crispri ... See more keywords
CRISPRi-mediated tunable control of gene expression level with engineered single-guide RNA in Escherichia coli
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad234
Abstract: Abstract Precise control of gene expression is essential for flux redistribution in metabolic pathways. Although the CRISPR interference (CRISPRi) system can effectively repress gene expression at the transcriptional level, it has still been difficult to… read more here.
Keywords: crispri; level; gene expression; gene ... See more keywords
CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.13382
Abstract: Targeted gene regulation is indispensable for reprogramming a cellular network to modulate a microbial phenotype. Here, we adopted the type II CRISPR interference (CRISPRi) system for simple and efficient regulation of target genes in Pseudomonas… read more here.
Keywords: gene regulation; gene; crispri; putida kt2440 ... See more keywords
Inducible CRISPRi-Based Operon Silencing and Selective in Trans Gene Complementation in Borrelia burgdorferi
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Bacteriology"
DOI: 10.1128/jb.00468-22
Abstract: Borrelia burgdorferi, the spirochetal bacterium causing Lyme disease, is a tick-borne pathogen of global importance. Here, we expand the genetic toolbox for studying B. burgdorferi physiology and pathogenesis by establishing a single plasmid-based, fully inducible,… read more here.
Keywords: system; crispri; physiology; borrelia burgdorferi ... See more keywords
A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02782-21
Abstract: Understanding the fundamental biology of malaria parasites through functional genetic/genomic studies is critical for identifying novel targets for antimalarial development. Conditional knockout/knockdown systems are required to study essential genes in the haploid blood stages of… read more here.
Keywords: system; crispri system; crispri; inducible crispri ... See more keywords
CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Microbial Cell Factories"
DOI: 10.1186/s12934-017-0802-x
Abstract: BackgroundMultiplex control of metabolic pathway genes is essential for maximizing product titers and conversion yields of fuels, chemicals, and pharmaceuticals in metabolic engineering. To achieve this goal, artificial transcriptional regulators, such as clustered regularly interspaced… read more here.
Keywords: competing pathway; pathway genes; repression; coli ... See more keywords
Generation and validation of versatile inducible CRISPRi embryonic stem cell and mouse model
Sign Up to like & getrecommendations! Published in 2020 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3000749
Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) 9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation of the epigenome and controlling of gene expression.… read more here.
Keywords: inducible crispri; crispri; expression; embryonic stem ... See more keywords