Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cell"
DOI: 10.1016/j.molcel.2017.09.012
Abstract: Summary Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a… read more here.
Keywords: based chemical; chemical genetic; combined crispri; genetic screens ... See more keywords
CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1104
Abstract: Abstract Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to maintaining multiple plasmids without… read more here.
Keywords: logic inverter; multiple plasmids; crispri based; inverter ... See more keywords