A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum
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DOI: 10.1128/spectrum.02782-21
Abstract: Understanding the fundamental biology of malaria parasites through functional genetic/genomic studies is critical for identifying novel targets for antimalarial development. Conditional knockout/knockdown systems are required to study essential genes in the haploid blood stages of… read more here.
Keywords: system; crispri system; crispri; inducible crispri ... See more keywords
A Study of Type II ɛ-PL Degrading Enzyme (pldII) in Streptomyces albulus through the CRISPRi System
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DOI: 10.3390/ijms23126691
Abstract: ε-Poly-L-lysine (ε-PL) is a widely used antibacterial peptide polymerized of 25–35 L-lysine residues. The antibacterial effect of ε-PL is closely related to the polymerization degree. However, the mechanism of ε-PL degradation in S. albulus remains… read more here.
Keywords: pldii; crispri system; enzyme pldii; repression ... See more keywords