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   Articles with "crrna" as a keyword



Integrating CRISPR-Cas12a with a crRNA-Mediated Catalytic Network for the Development of a Modular and Sensitive Aptasensor.

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recommendations! Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.2c00224

Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a, which exhibits excellent target DNA-activated trans-cleavage activity under the guidance of a programmable CRISPR RNA (crRNA), has shown great promise in next-generation biosensing technology. However, current CRISPR-Cas12a-based biosensors… read more here.

Keywords: network; modular sensitive; crrna; cas12a ... See more keywords

One-Pot Detection of Biomarker Apurinic/Apyrimidinic Endonuclease 1 Based on the Modified-crRNA Regulated Trans-Cleavage Activity of CRISPR/Cas12a.

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recommendations! Published in 2025 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.5c00335

Abstract: Apurinic/apyrimidinic endonuclease 1 (APE1), a critical protein in DNA repair, plays indispensable roles in the maintenance of cellular homeostasis, thereby garnering significant attention as a biomarker and therapeutic target for various disorders. Current APE1 sensing… read more here.

Keywords: detection; trans cleavage; apyrimidinic endonuclease; apurinic apyrimidinic ... See more keywords

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

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recommendations! Published in 2019 at "Nature Communications"

DOI: 10.1038/s41467-019-10507-3

Abstract: Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report the detailed structural and functional analysis of the uncultured Ruminococcus sp.… read more here.

Keywords: rna; crrna; cas13d; target cleavage ... See more keywords

The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system

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recommendations! Published in 2022 at "RNA Biology"

DOI: 10.1080/15476286.2022.2150812

Abstract: ABSTRACT CRISPR systems elicit interference when a foreign nucleic acid is detected by its ability to base-pair to crRNA. Understanding what degree of complementarity between a foreign nucleic acid and crRNA is required for interference… read more here.

Keywords: type iii; crrna; interference; crispr systems ... See more keywords

CRISPR RNA binding drives structural ordering that primes Cas7-11 for target cleavage

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recommendations! Published in 2025 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkaf271

Abstract: Abstract Type III-E CRISPR–Cas effectors, referred to as Cas7-11 or giant Repeat-Associated Mysterious Protein, are single proteins that cleave target RNAs (tgRNAs) without nonspecific collateral cleavage, opening new possibilities for RNA editing. Here, biochemical assays… read more here.

Keywords: cas7; crrna; target; crispr rna ... See more keywords

Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage

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recommendations! Published in 2023 at "PLOS Biology"

DOI: 10.1371/journal.pbio.3002023

Abstract: Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR–Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well… read more here.

Keywords: dna; crrna; apo cas12a; lb2cas12a crrna ... See more keywords