Integrating CRISPR-Cas12a with a crRNA-Mediated Catalytic Network for the Development of a Modular and Sensitive Aptasensor.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00224
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a, which exhibits excellent target DNA-activated trans-cleavage activity under the guidance of a programmable CRISPR RNA (crRNA), has shown great promise in next-generation biosensing technology. However, current CRISPR-Cas12a-based biosensors… read more here.
Keywords: network; modular sensitive; crrna; cas12a ... See more keywords
Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
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DOI: 10.1038/s41467-019-10507-3
Abstract: Cas13d, the type VI-D CRISPR-Cas effector, is an RNA-guided ribonuclease that has been repurposed to edit RNA in a programmable manner. Here we report the detailed structural and functional analysis of the uncultured Ruminococcus sp.… read more here.
Keywords: rna; crrna; cas13d; target cleavage ... See more keywords
The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
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DOI: 10.1080/15476286.2022.2150812
Abstract: ABSTRACT CRISPR systems elicit interference when a foreign nucleic acid is detected by its ability to base-pair to crRNA. Understanding what degree of complementarity between a foreign nucleic acid and crRNA is required for interference… read more here.
Keywords: type iii; crrna; interference; crispr systems ... See more keywords
Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage
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DOI: 10.1371/journal.pbio.3002023
Abstract: Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR–Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well… read more here.
Keywords: dna; crrna; apo cas12a; lb2cas12a crrna ... See more keywords