Cry3Aa*SpyCatcher Fusion Crystals Produced in Bacteria as Scaffolds for Multienzyme Coimmobilization.
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DOI: 10.1021/acs.bioconjchem.2c00003
Abstract: The production of Cry3Aa enzyme fusion crystals in Bacillus thuringiensis provides a direct method to immobilize individual enzymes and thereby improve their stability and recyclability. Nevertheless, many reactions require multiple enzymes to produce a desired… read more here.
Keywords: cry3aa; fusion crystals; coimmobilization; cry3aa spycat2 ... See more keywords
Identification and Characterization of Aminopeptidase-N as a Binding Protein for Cry3Aa in the Midgut of Monochamus alternatus (Coleoptera: Cerambycidae)
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Economic Entomology"
DOI: 10.1093/jee/toaa130
Abstract: Abstract Bacillus thuringiensis Cry proteins have been widely used over the past decades for many different insect pests, which are safe for users and the environment. The coleopteran-specific Cry3Aa toxin from B. thuringiensis exhibits toxicity… read more here.
Keywords: cry3aa; midgut; monochamus alternatus; binding protein ... See more keywords
Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil
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DOI: 10.1186/s13068-019-1509-5
Abstract: BackgroundWe have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from Bacillus thuringiensis.… read more here.
Keywords: biodiesel; cry3aa; waste cooking; genetically encoded ... See more keywords
Cry3Aa Toxin Is Not Suitable to Control Lepidopteran Pest Spodoptera littoralis (Boisd.)
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DOI: 10.3390/plants11101312
Abstract: The toxicity of the Bacillus thuringiensis (Bt) toxin Cry3Aa—originally used against the main potato pest, the Colorado potato beetle, Leptinotarsa decemlineata—was verified on this species and then evaluated against the Egyptian armyworm, Spodoptera littoralis, which… read more here.
Keywords: spodoptera littoralis; cry3aa; control; toxin ... See more keywords