Cryo‐electron tomography—the cell biology that came in from the cold
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12757
Abstract: Cryo‐electron tomography (cryo‐ET) provides high‐resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct electron detectors, the Volta phase plate and cryo‐focused ion beam milling have dramatically pushed image quality… read more here.
Keywords: cryo electron; cell biology; biology; electron tomography ... See more keywords
Guide for determination of protein structural ensembles by combining cryo-EM data with metadynamics.
Sign Up to like & getrecommendations! Published in 2022 at "FEBS open bio"
DOI: 10.1002/2211-5463.13542
Abstract: Metadynamics Electron Microscopy MetaInference (MEMMI) is an integrative structural biology method that enables a rapid and accurate characterization of protein structural dynamics at the atomic level and the error in the Cryo-EM experimental data, even… read more here.
Keywords: structural ensembles; microscopy; cryo; protein structural ... See more keywords
The cryo‐EM structure of full‐length RAD52 protein contains an undecameric ring
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13565
Abstract: The human RAD52 protein, which forms an oligomeric ring structure, is involved in DNA double‐strand break repair. The N‐terminal half of RAD52 is primarily responsible for self‐oligomerisation and DNA binding. Crystallographic studies have revealed the… read more here.
Keywords: terminal half; rad52 protein; structure; cryo ... See more keywords
Quantitative Cryo-Scanning Transmission Electron Microscopy of Biological Materials.
Sign Up to like & getrecommendations! Published in 2018 at "Advanced materials"
DOI: 10.1002/adma.201706681
Abstract: Electron tomography provides a detailed view into the 3D structure of biological cells and tissues. Physical fixation by vitrification of the aqueous medium provides the most faithful preservation of biological specimens in the native, fully… read more here.
Keywords: microscopy; electron microscopy; cryo; scanning transmission ... See more keywords
Atomic modeling of the ITS2 ribosome assembly subcomplex from cryo‐EM together with mass spectrometry‐identified protein–protein crosslinks
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3045
Abstract: The assembly of ribosomal subunits starts in the nucleus, initiated by co‐transcriptional folding of nascent ribosomal RNA (rRNA) transcripts and binding of ribosomal proteins and assembly factors. The internal transcribed spacer 2 (ITS2) is a… read more here.
Keywords: mass spectrometry; ribosome assembly; modeling its2; assembly ... See more keywords
Cryo-electron microscopy and cryo-electron tomography of nanoparticles.
Sign Up to like & getrecommendations! Published in 2017 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1417
Abstract: Cryo-transmission electron microscopy (cryo-TEM or cryo-EM) and cryo-electron tomography (cryo-ET) offer robust and powerful ways to visualize nanoparticles. These techniques involve imaging of the sample in a frozen-hydrated state, allowing visualization of nanoparticles essentially as… read more here.
Keywords: cryo electron; cryo tem; cryo; microscopy ... See more keywords
Getting Started with In Situ Cryo-Electron Tomography.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0966-8_1
Abstract: Cryo-electron tomography (cryo-ET) is an extremely powerful tool which is used to image cellular features in their close-to-native environment at a resolution where both protein structure and membrane morphology can be revealed. Compared to conventional… read more here.
Keywords: cryo electron; cryo; biology; electron tomography ... See more keywords
Single-Particle Cryo-EM of Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1394-8_9
Abstract: In the recent years, the protein databank has been fueled by the exponential growth of high-resolution electron cryo-microscopy (cryo-EM) structures. This trend will be further accelerated through the continuous software and method developments and the… read more here.
Keywords: high resolution; membrane proteins; biology; single particle ... See more keywords
Chemosensitization of hepatocellular carcinoma cells to sorafenib by β-caryophyllene oxide-induced inhibition of ABC export pumps
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Toxicology"
DOI: 10.1007/s00204-019-02395-9
Abstract: Several ATP-binding cassette (ABC) proteins reduce intracellular concentrations of antitumor drugs and hence weaken the response of cancer cells to chemotherapy. Accordingly, the inhibition of these export pumps constitutes a promising strategy to chemosensitize highly… read more here.
Keywords: hepatocellular carcinoma; cells sorafenib; export pumps; caryophyllene oxide ... See more keywords
The 2017 Nobel Prize in Chemistry: cryo-EM comes of age
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-0899-8
Abstract: The 2017 Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson for “developing cryo-electron microscopy (cryo-EM) for the high-resolution structure determination of biomolecules in solution.” This feature article summarizes some… read more here.
Keywords: 2017 nobel; chemistry; prize chemistry; chemistry cryo ... See more keywords
Cryo-EM structures of tau filaments from Alzheimer’s disease with PET ligand APN-1607
Sign Up to like & getrecommendations! Published in 2021 at "Acta Neuropathologica"
DOI: 10.1007/s00401-021-02294-3
Abstract: Tau and Aβ assemblies of Alzheimer’s disease (AD) can be visualized in living subjects using positron emission tomography (PET). Tau assemblies comprise paired helical and straight filaments (PHFs and SFs). APN-1607 (PM-PBB3) is a recently… read more here.
Keywords: tau; alzheimer disease; tau filaments; apn 1607 ... See more keywords