Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
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DOI: 10.1107/s2052252520000081
Abstract: Methods are presented that estimate symmetrical and antisymmetrical optical aberrations, as well as magnification anisotropy, in a cryo-EM data set. Considering these effects improves the resolution of the 3D reconstruction when these effects are present. read more here.
Keywords: order aberrations; magnification; cryo data; high order ... See more keywords
Towards automating single-particle cryo-EM data acquisition
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DOI: 10.1107/s2052252522012039
Abstract: Target selection for single-particle cryo-EM data acquisition sessions is mostly done manually by human operators, which is time consuming and leads to the inefficient use of instruments. The software toolbox Ptolemy [Kim et al. (2023).… read more here.
Keywords: data acquisition; cryo data; cryo; single particle ... See more keywords
Cryo-EM data collection, refinement, and validation statistics.
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DOI: 10.1371/journal.pbio.3000780.s012
Abstract: Overview of cryo-EM data collection, data processing, and model-fitting parameters for yeast native and reconstituted Lso2–80S complexes and for human CCDC124–EBP1 and SERBP1–eEF2–EBP1 complexes. CCDC124, coiled-coil domain containing short open reading frame 124; cryo-EM, cryo-electron… read more here.
Keywords: data collection; collection refinement; cryo data; refinement validation ... See more keywords