Cryo‐electron tomography—the cell biology that came in from the cold
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12757
Abstract: Cryo‐electron tomography (cryo‐ET) provides high‐resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct electron detectors, the Volta phase plate and cryo‐focused ion beam milling have dramatically pushed image quality… read more here.
Keywords: cryo electron; cell biology; biology; electron tomography ... See more keywords
Cryo‐electron microscopy of Na+,K+‐ATPase reveals how the extracellular gate locks in the E2·2K+ state
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14437
Abstract: Na+,K+‐ATPase (NKA) is one of the most important members of the P‐type ion‐translocating ATPases and plays a pivotal role in establishing electrochemical gradients for Na+ and K+ across the cell membrane. Presented here is a… read more here.
Keywords: state; electron microscopy; microscopy; microscopy atpase ... See more keywords
Cryo‐Electron Tomography of Toxoplasma gondii Indicates That the Conoid Fiber May Be Derived from Microtubules
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Science"
DOI: 10.1002/advs.202206595
Abstract: Toxoplasma gondii (T. gondii) is the causative agent of toxoplasmosis and can infect numerous warm‐blooded animals. An improved understanding of the fine structure of this parasite can help elucidate its replication mechanism. Previous studies have… read more here.
Keywords: gondii; conoid fiber; toxoplasma gondii; electron tomography ... See more keywords
Cryo-electron microscopy and cryo-electron tomography of nanoparticles.
Sign Up to like & getrecommendations! Published in 2017 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1417
Abstract: Cryo-transmission electron microscopy (cryo-TEM or cryo-EM) and cryo-electron tomography (cryo-ET) offer robust and powerful ways to visualize nanoparticles. These techniques involve imaging of the sample in a frozen-hydrated state, allowing visualization of nanoparticles essentially as… read more here.
Keywords: cryo electron; cryo tem; cryo; microscopy ... See more keywords
Getting Started with In Situ Cryo-Electron Tomography.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0966-8_1
Abstract: Cryo-electron tomography (cryo-ET) is an extremely powerful tool which is used to image cellular features in their close-to-native environment at a resolution where both protein structure and membrane morphology can be revealed. Compared to conventional… read more here.
Keywords: cryo electron; cryo; biology; electron tomography ... See more keywords
Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1406-8_13
Abstract: Cryo-electron microscopy has established as a mature structural biology technique to elucidate the three-dimensional structure of biological macromolecules. The Coulomb potential of the sample is imaged by an electron beam, and fast semi-conductor detectors produce… read more here.
Keywords: cryo electron; microscopy; electron microscopy; biology ... See more keywords
Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7759-8_4
Abstract: With the advent of direct electron detectors, cryo-EM has become a popular choice for molecular structure determination. Among its advantages over X-ray crystallography are (1) no need for crystals, (2) much smaller sample volumes, and… read more here.
Keywords: cryo electron; microscopy; time; resolved cryo ... See more keywords
Preparation of Proteins and Macromolecular Assemblies for Cryo-electron Microscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9869-2_13
Abstract: Cryo-electron microscopy has become popular as the penultimate step on the road to structure determination for many proteins and macromolecular assemblies. The process of obtaining high-resolution images of a purified biomolecular complex in an electron… read more here.
Keywords: cryo electron; proteins macromolecular; microscopy; macromolecular assemblies ... See more keywords
Visualizing Infection Initiation of Bacteriophage P22 by Cryo-Electron Tomography
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1704
Abstract: For successful infection, bacteriophages must overcome multiple barriers in the bacterial cell envelope to translocate viral DNA and proteins into the host cell. However, the molecular mechanisms underlying the phage infection initiation remain poorly understood.… read more here.
Keywords: cryo electron; phage; infection; p22 ... See more keywords
Cryo-Electron Tomography, Faster: Development of a Fast-Incremental Tilting Scheme for Rapid Tomogram Acquisition
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2018.11.3091
Abstract: Cryo-electron microscopy enables high-resolution imaging for both protein structure determination and cellular imaging studies. When a sample's ultrastructure is unique, cryo-electron tomography can generate a volume image from a single sample, allowing the study of… read more here.
Keywords: resolution; acquisition; microscopy; electron ... See more keywords
Structural insights into thermostabilization of leucine dehydrogenase from its atomic structure by cryo-electron microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2018.12.001
Abstract: Leucine dehydrogenase (LDH, EC 1.4.1.9) is a NAD+-dependent oxidoreductase that catalyzes the deamination of branched-chain l-amino acids (BCAAs). LDH of Geobacillus stearothermophilus (GstLDH) is a highly thermostable enzyme that has been applied for the quantification… read more here.
Keywords: cryo electron; microscopy; leucine dehydrogenase; electron microscopy ... See more keywords