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Published in 2017 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927617004883
Abstract: In recent years, biological specimen preparation using a cryo-focused ion beam (cryo-FIB) has become a key technique for investigating complex cellular structures in situ [1-3]. Cryo-FIB has enabled cryoelectron tomography (cryo-ET) of plunge-frozen hydrated cells,…
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Keywords:
preparation using;
fib lift;
cryo fib;
cryo ... See more keywords
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Published in 2019 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927619005816
Abstract: Understanding cellular ultrastructure and function, ideally in the native state requires a powerful analytical method. Cryo-electron tomography (cryo-ET) has become a well-established tool to study frozen vitrified specimens in a close-to-native state. A large proportion…
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Keywords:
workflow fabricating;
fib workflow;
fabricating thin;
cryo ... See more keywords
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Published in 2020 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927620020425
Abstract: The liquid-solid interface plays an essential role in many phenomena encountered in biological, chemical, and physical processes relevant to both fundamental and applied science [1]. However, hydrated materials, or more generally liquids, present a technical…
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Keywords:
sensitive materials;
microscopy;
fib specimen;
environmentally sensitive ... See more keywords
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Published in 2022 at "Nanotechnology"
DOI: 10.1088/1361-6528/ac92eb
Abstract: Primarily driven by structural biology, the rapid advances in cryogenic electron microscopy techniques are now being adopted and applied by materials scientists. Samples that inherently have electron transparency can be rapidly frozen (vitrified) in amorphous…
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Keywords:
tem;
beam;
microscopy;
cryo fib ... See more keywords