Cryo-EM structures reveal distinct apo conformations of sortilin-related receptor SORLA.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2022.01.108
Abstract: Sorting-related receptor with A-type repeats (SORLA) is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the structures of the full-length SORLA and identified two distinct conformations of apo-SORLA using… read more here.
Keywords: reveal distinct; distinct apo; structures reveal; cryo structures ... See more keywords
Atomic cryo-EM structures of viruses.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2017.07.002
Abstract: During the development of single particle cryo-EM in past five decades, icosahedral viruses have led the resolution progress owing to their large mass and high symmetry. Many technical advances in cryo-EM were first established with… read more here.
Keywords: resolution; virology; structures viruses; atomic cryo ... See more keywords
Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism
Sign Up to like & getrecommendations! Published in 2020 at "Cell Research"
DOI: 10.1038/s41422-020-0280-2
Abstract: The pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1R) belongs to the secretin receptor family and is widely distributed in the central neural system and peripheral organs. Abnormal activation of the receptor mediates trigeminovascular activation… read more here.
Keywords: mechanism; ligand binding; cryo structures; receptor ... See more keywords
A “Build and Retrieve” methodology to simultaneously solve cryo-EM structures of membrane proteins
Sign Up to like & getrecommendations! Published in 2021 at "Nature methods"
DOI: 10.1038/s41592-020-01021-2
Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we… read more here.
Keywords: build retrieve; methodology; membrane proteins; cryo structures ... See more keywords
Rcf2 revealed in cryo-EM structures of hypoxic isoforms of mature mitochondrial III-IV supercomplexes
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1920612117
Abstract: Significance As the terminal electron acceptor of our mitochondrial respiratory chains, complex IV drives and regulates oxidative phosphorylation, the process by which most of our ATP is produced. Complex IV forms supercomplexes (SCs) of different… read more here.
Keywords: iii; structures hypoxic; isoform; revealed cryo ... See more keywords
Cryo-EM structures of alphavirus conformational intermediates in low pH–triggered prefusion states
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2114119119
Abstract: Significance Eastern equine encephalitis virus (EEEV) is a neuroinvasive alphavirus that causes severe neurological diseases. Entry of alphaviruses requires host–virus membrane fusion in endosomes to release the RNA genome into the cytosol for virus replication.… read more here.
Keywords: fusion; membrane fusion; cryo structures; alphavirus conformational ... See more keywords
Cryo-EM structures of wild-type and E138K/M184I mutant HIV-1 RT/DNA complexed with inhibitors doravirine and rilpivirine
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2203660119
Abstract: Significance The enzyme reverse transcriptase (RT) is a key antiviral target, and nonnucleoside RT inhibitors (NNRTIs) are among the frequently used components of antiretroviral therapy for treating HIV-1 infection. The emergence of drug-resistant mutations continues… read more here.
Keywords: cryo structures; dna; m184i; hiv ... See more keywords
Cryo-EM structures of light-harvesting 2 complexes from Rhodopseudomonas palustris reveal the molecular origin of absorption tuning
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2210109119
Abstract: Significance The light-harvesting (LH) complexes of phototrophic bacteria absorb solar energy for photosynthesis, and it is important to understand how the protein components influence the way bound pigments absorb light. We studied the LH2 complexes… read more here.
Keywords: cryo structures; complexes rhodopseudomonas; harvesting complexes; structures light ... See more keywords
SNARE assembly enlightened by cryo-EM structures of a synaptobrevin–Munc18-1–syntaxin-1 complex
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1101/2022.03.05.483126
Abstract: Munc18-1 forms a template to recruit and organize assembly of the SNARE complex that triggers neurotransmitter release, binding first to a closed conformation of syntaxin-1 where its N-terminal region interacts with the SNARE motif, and… read more here.
Keywords: snare; cryo structures; syntaxin; complex assembly ... See more keywords
Cryo-EM structures of peripherin-2 and ROM1 suggest multiple roles in photoreceptor membrane morphogenesis
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.add3677
Abstract: Mammalian peripherin-2 (PRPH2) and rod outer segment membrane protein 1 (ROM1) are retina-specific tetraspanins that partake in the constant renewal of stacked membrane discs of photoreceptor cells that enable vision. Here, we present single-particle cryo–electron… read more here.
Keywords: rom1 suggest; structures peripherin; cryo structures; peripherin rom1 ... See more keywords
High-resolution cryo-EM structures of plant cytochrome b6f at work
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.add9688
Abstract: Plants use solar energy to power cellular metabolism. The oxidation of plastoquinol and reduction of plastocyanin by cytochrome b6f (Cyt b6f) is known as one of the key steps of photosynthesis, but the catalytic mechanism… read more here.
Keywords: resolution cryo; cryo structures; cytochrome b6f; high resolution ... See more keywords