Electron cryo-tomography of vestibular hair-cell stereocilia.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2019.02.006
Abstract: High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a… read more here.
Keywords: hair cell; cell stereocilia; cryo tomography; electron cryo ... See more keywords
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.02.28.482229
Abstract: We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised… read more here.
Keywords: cryo tomography; cryo; electron cryo; single particle ... See more keywords
Analysis of Cell–Cell Bridges in Haloferax volcanii Using Electron Cryo-Tomography Reveal a Continuous Cytoplasm and S-Layer
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.612239
Abstract: Halophilic archaea have been proposed to exchange DNA and proteins using a fusion-based mating mechanism. Scanning electron microscopy previously suggested that mating involves an intermediate state, where cells are connected by an intercellular bridge. To… read more here.
Keywords: continuous cytoplasm; microscopy; layer; cryo tomography ... See more keywords
Cryo-EM tomography and automatic segmentation delineate modular structures in the postsynaptic density
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Synaptic Neuroscience"
DOI: 10.3389/fnsyn.2023.1123564
Abstract: Postsynaptic densities (PSDs) are large protein complexes associated with the postsynaptic membrane of excitatory synapses important for synaptic function including plasticity. Conventional electron microscopy (EM) typically depicts PSDs as compact disk-like structures of hundreds of… read more here.
Keywords: psds; cryo tomography; microscopy; size ... See more keywords