Cryoelectron Microscopy Structure of a Yeast Centromeric Nucleosome at 2.7 Å Resolution.
Sign Up to like & getrecommendations! Published in 2020 at "Structure"
DOI: 10.1016/j.str.2019.12.002
Abstract: Kinetochores mediate chromosome segregation during cell division. They assemble on centromeric nucleosomes and capture spindle microtubules. In budding yeast, a kinetochore links a single nucleosome, containing the histone variant Cse4CENP-A instead of H3, with a… read more here.
Keywords: yeast centromeric; microscopy; cryoelectron microscopy; centromeric nucleosome ... See more keywords
Macromolecular refinement of X-ray and cryoelectron microscopy structures with Phenix/OPLS3e for improved structure and ligand quality.
Sign Up to like & getrecommendations! Published in 2021 at "Structure"
DOI: 10.1016/j.str.2021.03.011
Abstract: With the advent of the resolution revolution in cryoelectron microscopy (cryo-EM), low-resolution refinement is common, and likewise increases the need for a reliable force field. Here, we report on the incorporation of the OPLS3e force… read more here.
Keywords: quality; microscopy; cryoelectron microscopy; refinement ... See more keywords
Cryoelectron microscopy of Na+,K+-ATPase in the two E2P states with and without cardiotonic steroids
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2123226119
Abstract: Significance The E2P state of Na+,K+-ATPase, in which the ATPase is phosphorylated and of low affinity to Na+ with the extracellular gate opened, shows different biochemical properties depending on whether the phosphate is transferred from… read more here.
Keywords: two e2p; cryoelectron microscopy; microscopy; atpase two ... See more keywords
Developing Graphene Grids for Cryoelectron Microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.937253
Abstract: Cryogenic electron microscopy (cryo-EM) single particle analysis has become one of the major techniques used to study high-resolution 3D structures of biological macromolecules. Specimens are generally prepared in a thin layer of vitrified ice using… read more here.
Keywords: cryoelectron microscopy; microscopy; high resolution; developing graphene ... See more keywords